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验证数据展示

  • IF Staining of HepG2 using CL488-83532-3

    IF Staining of HepG2 using CL488-83532-3

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 SYNPO2L antibody (CL488-83532-3, Clone: 240523F12 ) at dilution of 1:200.

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CoraLite® Plus 488-conjugated SYNPO2L Recombinant monoclonal antibody

SYNPO2L Uni-rAb® Recombinant Antibody for IF/ICC
Cat No. CL488-83532-3

产品说明书

CloneNo. 240523F12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC

别名

240523F12, Synaptopodin 2 like protein, Synaptopodin 2-like protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 83532-3-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-83532-3 targets SYNPO2L in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原

CatNo: Ag34027

Product name: Recombinant human SYNPO2L protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 35-389 aa of BC142635

Sequence: LQRAESLQEKSIKEAKTKCRTIASLLTAAPNPHSKGVLMFKKRRQRAKKYTLVSFGAAAGTGAEEEDGVPPTSESELDEEAFSDARSLTNQSDWDSPYLDMELARAGSRASEGQGSGLGGQLSEVSGRGVQLFEQQRQRADSSTQELARVEPAAMLNGEGLQSPPRAQSAPPEAAVLPPSPLPAPVASPRPFQPGGGAPTPAPSIFNRSARPFTPGLQGQRPTTTSVIFRPLAPKRANDSLGGLSPAPPPFLSSQGPTPLPSFTSGVPSHAPVSGSPSTPRSSGPVTATSSLYIPAPSRPVTPGGAPEPPAPPSAAAMTSTASIFLSAPLRPSARPEAPAPGPGAPEPPSAREQR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 synaptopodin 2-like
别名 240523F12, Synaptopodin 2 like protein, Synaptopodin 2-like protein
计算分子量 977 aa, 102 kDa
GenBank蛋白编号BC142635
基因名称 SYNPO2L
Gene ID (NCBI) 79933
RRIDAB_3673267
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9H987
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SYNPO2L belongs to the synaptopodin family and is an actin-associated protein that may play a role in modulating actin-based shape. SYNPO2L is involved in the positive regulation of Rho protein signal transduction and the positive regulation of stress fiber assembly. Loss-of-function variants in the SYNPO2L gene are associated with atrial fibrillation (PMID: 33768119).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 SYNPO2L antibody CL488-83532-3Download protocol
Standard Protocols
Click here to view our Standard Protocols