验证数据展示
经过测试的应用
| Positive IF/ICC detected in | U-251 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-85045 targets SYNGAP1 in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | synaptic Ras GTPase activating protein 1 homolog (rat) |
| 别名 | 242255E3, Neuronal RasGAP, Ras GTPase activating protein SynGAP, Ras/Rap GTPase-activating protein SynGAP, RASA 1 |
| 计算分子量 | 1343 aa, 148 kDa |
| 观测分子量 | 140 kDa |
| GenBank蛋白编号 | NM_006772 |
| 基因名称 | SYNGAP1 |
| Gene ID (NCBI) | 8831 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q96PV0 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SYNGAP1, also named as KIAA1938, is the major constituent of the PSD essential for postsynaptic signaling. It's an inhibitory regulator of the Ras-cAMP pathway. SYNGAP1 is a member of the NMDAR signaling complex in excitatory synapses, it may play a role in NMDAR-dependent control of AMPAR potentiation, AMPAR membrane trafficking and synaptic plasticity. SYNGAP1 regulates AMPAR-mediated miniature excitatory postsynaptic currents. SYNGAP1 may be involved in certain forms of brain injury, leading to long-term learning and memory deficits Defects in SYNGAP1 are the cause of mental retardation autosomal dominant type 5 (MRD5).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 SYNGAP1 antibody CL488-85045 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |