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验证数据展示

  • WB analysis using 83331-1-RR (same clone as 83331-1-PBS)

    WB analysis using 83331-1-RR (same clone as 83331-1-PBS)

    Non-treated Ramos cells, and pervanadate treated Ramos cells were subjected to SDS PAGE followed by western blot with 83331-1-RR (Phospho-SYK (Tyr323) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as loading control. This data was developed using the same antibody clone with 83331-1-PBS in a different storage buffer formulation.

  • IF Staining of Ramos using 83331-1-RR (same clone as 83331-1-PBS)

    IF Staining of Ramos using 83331-1-RR (same clone as 83331-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed pervanadate treated Ramos cells using Phospho-SYK (Tyr323) antibody (83331-1-RR, Clone: 2H24 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83331-1-PBS in a different storage buffer formulation.

Phospho-SYK (Tyr323) Recombinant monoclonal antibody, PBS Only

Phospho-SYK (Tyr323) Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 83331-1-PBS

产品说明书

CloneNo. 2H24

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

p SYK, p SYK (Tyr323), p SYK Tyr323, Phospho SYK, Phospho SYK (Tyr323)

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83331-1-PBS targets Phospho-SYK (Tyr323) in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 spleen tyrosine kinase
别名 p SYK, p SYK (Tyr323), p SYK Tyr323, Phospho SYK, Phospho SYK (Tyr323)
计算分子量 72 kDa
GenBank蛋白编号BC001645
基因名称 SYK
Gene ID (NCBI) 6850
ENSEMBL Gene IDENSG00000165025
RRIDAB_3670995
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP43405
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SYK(spleen tyrosine kinase) is a key regulator of signal transduction events,apoptosis and orderly cell cycle progression in B-lineage lymphoid cells. The study found that in human platelets, inhibition of PKC leads to Syk hyperphosphorylation on residues Tyr-525/526 whereas Tyr-323 and Tyr-352 phosphorylations are unaffected. PKC negatively regulates Syk activity, as evidenced by hyperphosphorylation of downstream targets, LAT and PLCγ2 upon PKC inhibition. (PMID: 23960082)

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