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验证数据展示

  • WB analysis of Ramos using 83331-1-RR

    WB analysis of Ramos using 83331-1-RR

    Non-treated Ramos cells, and pervanadate treated Ramos cells were subjected to SDS PAGE followed by western blot with 83331-1-RR (Phospho-SYK (Tyr323) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as loading control.

  • IF Staining of Ramos using 83331-1-RR

    IF Staining of Ramos using 83331-1-RR

    Immunofluorescent analysis of (4% PFA) fixed pervanadate treated Ramos cells using Phospho-SYK (Tyr323) antibody (83331-1-RR, Clone: 2H24 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Phospho-SYK (Tyr323) Recombinant monoclonal antibody

Phospho-SYK (Tyr323) Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 83331-1-RR

产品说明书

CloneNo. 2H24
引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IF/ICC, ELISA

别名

p SYK, p SYK (Tyr323), p SYK Tyr323, Phospho SYK, Phospho SYK (Tyr323)

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inpervanadate treated Ramos cells
Positive IF/ICC detected inpervanadate treated Ramos cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

83331-1-RR targets Phospho-SYK (Tyr323) in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性mouse
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 spleen tyrosine kinase
别名 p SYK, p SYK (Tyr323), p SYK Tyr323, Phospho SYK, Phospho SYK (Tyr323)
计算分子量 72 kDa
观测分子量70 kDa
GenBank蛋白编号BC001645
基因名称 SYK
Gene ID (NCBI) 6850
ENSEMBL Gene IDENSG00000165025
RRIDAB_3670995
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP43405
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SYK(spleen tyrosine kinase) is a key regulator of signal transduction events,apoptosis and orderly cell cycle progression in B-lineage lymphoid cells. The study found that in human platelets, inhibition of PKC leads to Syk hyperphosphorylation on residues Tyr-525/526 whereas Tyr-323 and Tyr-352 phosphorylations are unaffected. PKC negatively regulates Syk activity, as evidenced by hyperphosphorylation of downstream targets, LAT and PLCγ2 upon PKC inhibition. (PMID: 23960082)

实验方案

Product Specific Protocols
IF protocol for Phospho-SYK (Tyr323) antibody 83331-1-RRDownload protocol
WB protocol for Phospho-SYK (Tyr323) antibody 83331-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

J Diabetes Investig

Linderalactone mitigates diabetic renal injury by inhibiting macrophage inflammation via the Dectin1/Syk/CARD9/IRF5/NF-κB pathway.

Authors - Hao Chen
View Article
mouseWB

Antioxidants (Basel)

Neuron-Derived Sema3B Facilitates Microglial Hematoma Clearance After Intracerebral Hemorrhage.

Authors - Baisong Huang
View Article
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