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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated SUMO2/3 Monoclonal antibody

SUMO2/3 Monoclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1A1B3

Cat No : CL594-67154

Print datasheet

Synonyms

HSMT3, Sentrin 2, SMT3 homolog 2, SMT3B, SMT3H2, SUMO 2, SUMO 3, SUMO2, SUMO2/3, Ubiquitin like protein SMT3B

验证数据展示

  • IF Staining of HeLa using CL594-67154

    IF Staining of HeLa using CL594-67154

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CL594-67154 (SUMO2/3 antibody) at dilution of 1:100.

  • FC experiment of HeLa using CL594-67154

    FC experiment of HeLa using CL594-67154

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human SUMO2/3 (CL594-67154, Clone:1A1B3) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IF Staining of HeLa using CL594-67154

    IF Staining of HeLa using CL594-67154

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 SUMO2/3 antibody (CL594-67154, Clone: 1A1B3 ) at dilution of 1:200.

  • FC experiment of HeLa using CL594-67154

    FC experiment of HeLa using CL594-67154

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human SUMO2/3 (CL594-67154, Clone:1A1B3) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF detected inHeLa cells
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67154-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67154 targets SUMO2/3 in IF, FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity Human, Mouse, Rat
Immunogen SUMO2/3 fusion protein Ag28672 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae)
Synonyms HSMT3, Sentrin 2, SMT3 homolog 2, SMT3B, SMT3H2, SUMO 2, SUMO 3, SUMO2, SUMO2/3, Ubiquitin like protein SMT3B
Calculated Molecular Weight 11 kDa
Observed Molecular Weight 18 kDa
GenBank Accession NumberBC008450
Gene Symbol SUMO2
Gene ID (NCBI) 6613
RRIDAB_2883641
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP61956
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ubiquitin is most famous for its function in targeting proteins for degradation by the 26S proteasome, ubiquitin needs to be attached to a substrate in chains (polyubiquitylation) before being recognized by proteasome. Similarly, SUMO (small ubiquitin-related modifier) can be linked to substrates in chains (polysumoylation), SUMO modification has been implicated in many important cellular processes including the control of genome stability, signal transduction, targeting to and formation of nuclear compartments, cell cycle and meiosis. There are 4 confirmed SUMO isoforms in human, SUMO-1, SUMO-2, SUMO-3 and SUMO-4. SUMO-2 and SUMO-3 are nearly identical but are distinct from SUMO-1. SUMO2/3 conjugation was recently widely involved in neuroprotective activities. A substitution (M55V) of SUMO4 was strongly associated with the pathogenesis of type 1 diabetes (T1D) involving NF kappa B related mechanisms.

实验方案

Product Specific Protocols
IF protocol for CL594 SUMO2/3 antibody CL594-67154Download protocol
FC protocol for CL594 SUMO2/3 antibody CL594-67154Download protocol
Standard Protocols
Click here to view our Standard Protocols