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验证数据展示

  • WB analysis of mouse liver using 12260-1-AP (same clone as 12260-1-PBS)

    WB analysis of mouse liver using 12260-1-AP (same clone as 12260-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 12260-1-AP (SULF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12260-1-PBS in a different storage buffer formulation.

  • WB analysis using 12260-1-AP (same clone as 12260-1-PBS)

    WB analysis using 12260-1-AP (same clone as 12260-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12260-1-AP (SULF2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12260-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse ovary using 12260-1-AP (same clone as 12260-1-PBS)

    IHC staining of mouse ovary using 12260-1-AP (same clone as 12260-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 12260-1-AP (SULF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12260-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SULF2 antibody included
  • IHC staining of mouse ovary using 12260-1-AP (same clone as 12260-1-PBS)

    IHC staining of mouse ovary using 12260-1-AP (same clone as 12260-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 12260-1-AP (SULF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12260-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SULF2 antibody included
  • IHC staining of mouse heart using 12260-1-AP (same clone as 12260-1-PBS)

    IHC staining of mouse heart using 12260-1-AP (same clone as 12260-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12260-1-AP (SULF2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12260-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SULF2 antibody included
  • Featured Product
  • KD/KO Validated

SULF2 Polyclonal antibody, PBS Only

SULF2 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 12260-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

Arylsulfatase, EC:3.1.6.1, EC:3.1.6.14, Extracellular sulfatase Sulf 2, Extracellular sulfatase Sulf-2

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy SULF2 Ready-To-Use IHC Kit. SULF2 primary antibody included.

产品信息

12260-1-PBS targets SULF2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2915

Product name: Recombinant human SULF2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 722-870 aa of BC020962

Sequence: MPGLTCFTHDNQHWQTAPFWTLGPFCACTSANNNTYWCMRTINETHNFLFCEFATGFLEYFDLNTDPYQLMNAVNTLDRDVLNQLHVQLMELRSCKGYKQCNPRTRNMDLGLKDGGSYEQYRQFQRRKWPEMKRPSSKSLGQLWEGWEG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sulfatase 2
别名 Arylsulfatase, EC:3.1.6.1, EC:3.1.6.14, Extracellular sulfatase Sulf 2, Extracellular sulfatase Sulf-2
计算分子量 870 aa, 96 kDa
观测分子量 50 kDa, 130-150 kDa
GenBank蛋白编号BC020962
基因名称 SULF2
Gene ID (NCBI) 55959
RRIDAB_11183762
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IWU5
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SULF2, also named as KIAA1247, belongs to the sulfatase family. It exhibits arylsulfatase activity and highly specific endoglucosamine-6-sulfatase activity. SULF2 can remove sulfate from the C-6 position of glucosamine within specific subregions of intact heparin. SULF2 has several forms: the unglycosylated 100 kDa SULF2, the glycosylated 120-150 kDa SULF2, the cleaved 72-75 kDa and 50 kDa SULF2.

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