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验证数据展示

  • IF Staining of HeLa using CL488-26759

    IF Staining of HeLa using CL488-26759

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 SUFU antibody (CL488-26759) at dilution of 1:200.

  • FC experiment of HEK-293 using CL488-26759

    FC experiment of HEK-293 using CL488-26759

    1x10^6 HEK-293 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human SUFU (CL488-26759) (red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated SUFU Polyclonal antibody

SUFU Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-26759

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

PRO1280, SUFUH, Suppressor of fused homolog, UNQ650/PRO1280

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy SUFU Ready-To-Use IHC Kit. SUFU primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 26759-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-26759 targets SUFU in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag25200

Product name: Recombinant human SUFU protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 185-454 aa of BC013291

Sequence: MQPVQTPFGVVTFLQIVGVCTEELHSAQQWNGQGILELLRTVPIAGGPWLITDMRRGETIFEIDPHLQERVDKGIETDGSNLSGVSAKCAWDDLSRPPEDDEDSRSICIGTQPRRLSGKDTEQIRETLRRGLEINSKPVLPPINPQRQNGLAHDRAPSRKDSLESDSSTAIIPHELIRTRQLESVHLKFNQESGALIPLCLRGRLLHGRHFTYKSITGDMAITFVSTGVEGAFATEEHPYAAHGPWLQILLTEEFVEKMLEDLEDLTSPE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 suppressor of fused homolog (Drosophila)
别名 PRO1280, SUFUH, Suppressor of fused homolog, UNQ650/PRO1280
计算分子量 54 kDa
观测分子量 50 kDa
GenBank蛋白编号BC013291
基因名称 SUFU
Gene ID (NCBI) 51684
RRIDAB_3084100
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UMX1
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sufu (Suppressor of fused) is a central regulator of Hh (Hedgehog) signaling and acts as a tumor suppressor by maintaining the Gli (Glioma-associated oncogene homolog) transcription factors inactive. Loss of Sufu function promotes tumorigenesis.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 SUFU antibody CL488-26759Download protocol
IF protocol for CL Plus 488 SUFU antibody CL488-26759Download protocol
Standard Protocols
Click here to view our Standard Protocols
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