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Biotin-conjugated STXBP1 Polyclonal antibody

STXBP1 Polyclonal Antibody for IHC, WB

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB

Conjugate

Biotin

Cat No : Biotin-11459

Print datasheet

Synonyms

EIEE4, FLJ37475, MUNC18 1, N Sec1, p67, Protein unc 18 homolog 1, Protein unc 18 homolog A, RBSEC1, STXBP1, syntaxin binding protein 1, Unc 18A, UNC18, Unc18 1, UNC18A

验证数据展示

  • WB analysis of Y79 using Biotin-11459

    WB analysis of Y79 using Biotin-11459

    Y79 cells were subjected to SDS PAGE followed by western blot with Biotin-11459 (STXBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using Biotin-11459

    IHC staining of mouse brain using Biotin-11459

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using Biotin-11459 (STXBP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using Biotin-11459

    IHC staining of mouse brain using Biotin-11459

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using Biotin-11459 (STXBP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inY79 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

Biotin-11459 targets STXBP1 in WB, IHC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB
Tested Reactivity human, mouse, rat
Immunogen STXBP1 fusion protein Ag1978 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name syntaxin binding protein 1
Synonyms EIEE4, FLJ37475, MUNC18 1, N Sec1, p67, Protein unc 18 homolog 1, Protein unc 18 homolog A, RBSEC1, STXBP1, syntaxin binding protein 1, Unc 18A, UNC18, Unc18 1, UNC18A
Calculated Molecular Weight 594 aa, 68 kDa
Observed Molecular Weight 65-68 kDa
GenBank Accession NumberBC015749
Gene Symbol STXBP1
Gene ID (NCBI) 6812
RRIDAB_2918937
Conjugate Biotin
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP61764
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Biotin STXBP1 antibody Biotin-11459Download protocol
IHC protocol for Biotin STXBP1 antibody Biotin-11459Download protocol
Standard Protocols
Click here to view our Standard Protocols