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验证数据展示

  • WB analysis of Y79 using Biotin-11459

    WB analysis of Y79 using Biotin-11459

    Y79 cells were subjected to SDS PAGE followed by western blot with Biotin-11459 (STXBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using Biotin-11459

    IHC staining of mouse brain using Biotin-11459

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using Biotin-11459 (STXBP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STXBP1 antibody included
  • IHC staining of mouse brain using Biotin-11459

    IHC staining of mouse brain using Biotin-11459

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using Biotin-11459 (STXBP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STXBP1 antibody included
  • Featured Product
  • KD/KO验证

Biotin-conjugated STXBP1 Polyclonal antibody

STXBP1 Polyclonal Antibody for WB, IHC
Cat No. Biotin-11459

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC

别名

EIEE4, FLJ37475, MUNC18 1, N Sec1, p67, Protein unc 18 homolog 1, Protein unc 18 homolog A, RBSEC1, STXBP1, syntaxin binding protein 1, Unc 18A, UNC18, Unc18 1, UNC18A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Biotin
规格价格库存

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经过测试的应用

Positive WB detected inY79 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy STXBP1 Ready-To-Use IHC Kit. STXBP1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

Biotin-11459 targets STXBP1 in WB, IHC applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1978

Product name: Recombinant human STXBP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 245-594 aa of BC015749

Sequence: HELTFQAMSYDLLPIENDVYKYETSGIGEARVKEVLLDEDDDLWIALRHKHIAEVSQEVTRSLKDFSSSKRMNTGEKTTMRDLSQMLKKMPQYQKELSKYSTHLHLAEDCMKHYQGTVDKLCRVEQDLAMGTDAEGEKIKDPMRAIVPILLDANVSTYDKIRIILLYIFLKNGITEENLNKLIQHAQIPPEDSEIITNMAHLGVPIVTDSTLRRRSKPERKERISEQTYQLSRWTPIIKDIMEDTIEDKLDTKHYPYISTRSSASFSTTAVSARYGHWHKNKAPGEYRSGPRLIIFILGGVSLNEMRCAYEVTQANGKWEVLIGSTHILTPQKLLDTLKKLNKTDEEISS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 syntaxin binding protein 1
别名 EIEE4, FLJ37475, MUNC18 1, N Sec1, p67, Protein unc 18 homolog 1, Protein unc 18 homolog A, RBSEC1, STXBP1, syntaxin binding protein 1, Unc 18A, UNC18, Unc18 1, UNC18A
计算分子量 594 aa, 68 kDa
观测分子量 65-68 kDa
GenBank蛋白编号BC015749
基因名称 STXBP1
Gene ID (NCBI) 6812
RRIDAB_2918937
偶联类型 Biotin
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP61764
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Biotin STXBP1 antibody Biotin-11459Download protocol
IHC protocol for Biotin STXBP1 antibody Biotin-11459Download protocol
Standard Protocols
Click here to view our Standard Protocols