验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse brain tissue |
| Positive IF/ICC detected in | NIH/3T3 cells |
| Positive FC (Intra) detected in | Daudi cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-83081-2 targets STK39 in IF/ICC, IF-P applications and shows reactivity with human, rat samples.
| 经测试应用 | IF/ICC, IF-P Application Description |
| 经测试反应性 | human, rat |
| 免疫原 |
CatNo: Ag34503 Product name: Recombinant human STK39 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 396-455 aa of BC166614 Sequence: GKAAFSQEKSRRVKEENPEIAVSASTIPEQIQSLSVHDSQGPPNANEDYREASSCAVNLV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | serine threonine kinase 39 (STE20/SPS1 homolog, yeast) |
| 别名 | 230382G9, DCHT, PASK, SPAK, Ste 20 related kinase |
| 计算分子量 | 59 kDa |
| 观测分子量 | 62 kDa |
| GenBank蛋白编号 | BC166614 |
| 基因名称 | STK39 |
| Gene ID (NCBI) | 27347 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9UEW8 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
STK39 (Serine/Threonine Kinase 39) is a proline- and alanine-rich Ste20-related kinase (also named as SPAK), which is composed of a short N-terminal proline and alanine repeats (PAPA box), a kinase catalytic domain and a C-terminal regulatory domain (PMID: 16382158). In mammalians, STK39 plays an important role in ion homeostasis by regulating the cation chloride cotransporters' activities, which is crucial for the modulation of renal salt transport and blood pressure (PMID: 33500714). Notably, STK39 is also implicated in the regulation of tumor cell proliferation, migration, and invasion in multiple cancers, including osteosarcoma and cervical cancer (PMID: 34335956).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 STK39 antibody CL488-83081-2 | Download protocol |
| IF protocol for CL Plus 488 STK39 antibody CL488-83081-2 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
