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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated STAM Polyclonal antibody

STAM Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-12434

Print datasheet

Synonyms

DKFZp686J2352, STAM, STAM 1, STAM1

验证数据展示

  • FC experiment of HeLa using CL488-12434

    FC experiment of HeLa using CL488-12434

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human STAM (CL488-12434) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 12434-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-12434 targets STAM in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra)
Tested Reactivity human, mouse, rat
Immunogen STAM fusion protein Ag3112 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name signal transducing adaptor molecule (SH3 domain and ITAM motif) 1
Synonyms DKFZp686J2352, STAM, STAM 1, STAM1
Calculated Molecular Weight 59 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC030586
Gene Symbol STAM
Gene ID (NCBI) 8027
RRIDAB_2919053
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92783
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

背景介绍

STAM, also named as STAM1, belongs to the STAM family. It is involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. STAM may also play a role in T-cell development. It is involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs). Together with HRS, STAM forms ESCRT-0. STAM was originally identified as an adaptor protein involved in cytokine signaling.(PMID:20505072)

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 STAM antibody CL488-12434Download protocol
Standard Protocols
Click here to view our Standard Protocols