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验证数据展示

  • WB analysis using 12434-1-AP

    WB analysis using 12434-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12434-1-AP (STAM antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IP experiment of PC-3 using 12434-1-AP

    IP experiment of PC-3 using 12434-1-AP

    IP result of anti-STAM (IP:12434-1-AP, 4ug; Detection:12434-1-AP 1:300) with PC-3 cells lysate 1440ug.

  • IHC staining of human testis using 12434-1-AP

    IHC staining of human testis using 12434-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12434-1-AP (STAM antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with STAM antibody included
  • IF Staining of HepG2 using 12434-1-AP

    IF Staining of HepG2 using 12434-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using STAM antibody (12434-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IHC staining of human testis using 12434-1-AP

    IHC staining of human testis using 12434-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12434-1-AP (STAM antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with STAM antibody included
  • IHC staining of mouse testis using 12434-1-AP

    IHC staining of mouse testis using 12434-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12434-1-AP (STAM antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STAM antibody included
  • IHC staining of mouse testis using 12434-1-AP

    IHC staining of mouse testis using 12434-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12434-1-AP (STAM antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STAM antibody included
  • FC experiment of HeLa using 12434-1-AP

    FC experiment of HeLa using 12434-1-AP

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug STAM Polyclonal antibody (12434-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

  • IHC staining of mouse kidney using 12434-1-AP

    IHC staining of mouse kidney using 12434-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12434-1-AP (STAM antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STAM antibody included
  • IHC staining of human appendicitis using 12434-1-AP

    IHC staining of human appendicitis using 12434-1-AP

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 12434-1-AP (STAM antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STAM antibody included
  • IHC staining of human rectal cancer using 12434-1-AP

    IHC staining of human rectal cancer using 12434-1-AP

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 12434-1-AP (STAM antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STAM antibody included
  • IHC staining of mouse cerebellum using 12434-1-AP

    IHC staining of mouse cerebellum using 12434-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12434-1-AP (STAM antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STAM antibody included
  • IHC staining of mouse small intestine using 12434-1-AP

    IHC staining of mouse small intestine using 12434-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 12434-1-AP (STAM antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STAM antibody included
  • IHC staining of rat kidney using 12434-1-AP

    IHC staining of rat kidney using 12434-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 12434-1-AP (STAM antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STAM antibody included
  • IHC staining of rat small intestine using 12434-1-AP

    IHC staining of rat small intestine using 12434-1-AP

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 12434-1-AP (STAM antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STAM antibody included
  • IHC staining of human rectal cancer using 12434-1-AP

    IHC staining of human rectal cancer using 12434-1-AP

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 12434-1-AP (STAM antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STAM antibody included
  • Featured Product
  • KD/KO Validated

STAM Polyclonal antibody

STAM Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 12434-1-AP

产品说明书

引用文献(22)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (2)

别名

STAM 1, STAM1, STAM-1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293T cells, Multi-cells, mouse brain tissue, Y79 cells, Jurkat cells, K-562 cells, MCF-7 cells, PC-3 cells, Raji cells, SKOV-3 cells, C2C12 cells
Positive IP detected inPC-3 cells
Positive IHC detected inhuman testis tissue, human appendicitis tissue, rat small intestine tissue, mouse small intestine tissue, mouse testis tissue, mouse cerebellum tissue, rat kidney tissue, human rectal cancer tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy STAM Ready-To-Use IHC Kit. STAM primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12434-1-AP targets STAM in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IF, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, monkey
免疫原

CatNo: Ag3112

Product name: Recombinant human STAM protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 54-403 aa of BC030586

Sequence: VNHKDPHVAMQALTLLGACVSNCGKIFHLEVCSRDFASEVSNVLNKGHPKVCEKLKALMVEWTDEFKNDPQLSLISAMIKNLKEQGVTFPAIGSQAAEQAKASPALVAKDPGTVANKKEEEDLAKAIELSLKEQRQQSTTLSTLYPSTSSLLTNHQHEGRKVRAIYDFEAAEDNELTFKAGEIITVLDDSDPNWWKGETHQGIGLFPSNFVTAYLTAEPEMIKTEKKTVQFSDDVQVETIEPEPEPAFIDEDKMDQLLQMLQSTDPSDDQPDLPELLHLEAMCHQMGPLIDEKLEDIDRKHSELSELNVKVMEALSLYTKLMNEDPMYSMYAKLQNQPGSGPTIRKPSPS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 signal transducing adaptor molecule (SH3 domain and ITAM motif) 1
别名 STAM 1, STAM1, STAM-1
计算分子量 59 kDa
观测分子量 70 kDa
GenBank蛋白编号BC030586
基因名称 STAM
Gene ID (NCBI) 8027
RRIDAB_2199965
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ92783
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STAM, also named as STAM1, belongs to the STAM family. It is involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. STAM may also play a role in T-cell development. It is involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs). Together with HRS, STAM forms ESCRT-0. STAM was originally identified as an adaptor protein involved in cytokine signaling.(PMID:20505072)

实验方案

Product Specific Protocols
FC protocol for STAM antibody 12434-1-APDownload protocol
IF protocol for STAM antibody 12434-1-APDownload protocol
IHC protocol for STAM antibody 12434-1-APDownload protocol
IP protocol for STAM antibody 12434-1-APDownload protocol
WB protocol for STAM antibody 12434-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nature

A phosphoinositide signalling pathway mediates rapid lysosomal repair

Authors - Jay Xiaojun Tan
View Article
humanIF

Nat Cell Biol

Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation.

Authors - Vincent Mercier
View Article
WB,IF

Nat Commun

Mapping of endosomal proximity proteomes reveals Retromer as a hub for RAB GTPase regulation

Authors - Carlos Antón-Plágaro
View Article
humanWB

Mol Cell

Systematically defining selective autophagy receptor-specific cargo using autophagosome content profiling.

Authors - Susanne Zellner
View Article
humanWB

Sci Adv

A ubiquitin-specific, proximity-based labeling approach for the identification of ubiquitin ligase substrates

Authors - Urbi Mukhopadhyay
View Article
humanWB,CoIP

Cell Rep

A MYC-STAMBPL1-TOE1 positive feedback loop mediates EGFR stability in hepatocellular carcinoma

Authors - Hongli Zhang
View Article
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