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CoraLite®555-conjugated SSRP1 Monoclonal antibody

SSRP1 Monoclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

3D3H4

Cat No : CL555-67313

Print datasheet

Synonyms

FACT, FACT 80 kDa subunit, FACT complex subunit SSRP1, FACT80, FACTp80, hSSRP1, SSRP1, T160

验证数据展示

  • IF Staining of HepG2 using CL555-67313

    IF Staining of HepG2 using CL555-67313

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®555 SSRP1 antibody (CL555-67313, Clone: 3D3H4 ) at dilution of 1:200.

  • FC experiment of HeLa using CL555-67313

    FC experiment of HeLa using CL555-67313

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human SSRP1 (CL555-67313, Clone:3D3H4) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy SSRP1 Ready-To-Use IHC Kit. SSRP1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67313-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-67313 targets SSRP1 in IF, FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity Human, mouse, rat
Immunogen SSRP1 fusion protein Ag8318 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name structure specific recognition protein 1
Synonyms FACT, FACT 80 kDa subunit, FACT complex subunit SSRP1, FACT80, FACTp80, hSSRP1, SSRP1, T160
Calculated Molecular Weight 81 kDa
Observed Molecular Weight 86 kDa
GenBank Accession NumberBC005116
Gene Symbol SSRP1
Gene ID (NCBI) 6749
RRIDAB_2919701
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ08945
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL555 SSRP1 antibody CL555-67313Download protocol
FC protocol for CL555 SSRP1 antibody CL555-67313Download protocol
Standard Protocols
Click here to view our Standard Protocols