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验证数据展示

  • IF Staining of HepG2 using CL555-67313

    IF Staining of HepG2 using CL555-67313

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®555 SSRP1 antibody (CL555-67313, Clone: 3D3H4 ) at dilution of 1:200.

  • IF Staining of HepG2 using CL555-67313

    IF Staining of HepG2 using CL555-67313

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®555 SSRP1 antibody (CL555-67313, Clone: 3D3H4 ) at dilution of 1:400, CL488-Phalloidin (green).

  • FC experiment of HeLa using CL555-67313

    FC experiment of HeLa using CL555-67313

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human SSRP1 (CL555-67313, Clone:3D3H4) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO Validated

CoraLite®555-conjugated SSRP1 Monoclonal antibody

SSRP1 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL555-67313

产品说明书

CloneNo. 3D3H4

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

3D3H4, Chromatin-specific transcription elongation factor 80 kDa subunit, Facilitates chromatin transcription complex 80 kDa subunit, Facilitates chromatin transcription complex subunit SSRP1, FACT

缓冲液配方:  PBS, Proclin300, BSA, Glycerol
PBS, Proclin300, BSA, Glycerol
偶联物:  CoraLite®555
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy SSRP1 Ready-To-Use IHC Kit. SSRP1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67313-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-67313 targets SSRP1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag8318

Product name: Recombinant human SSRP1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 361-709 aa of BC005116

Sequence: YVHKPPVHIRFDEISFVNFARGTTTTRSFDFEIETKQGTQYTFSSIEREEYGKLFDFVNAKKLNIKNRGLKEGMNPSYDEYADSDEDQHDAYLERMKEEGKIREENANDSSDDSGEETDESFNPGEEEEDVAEEFDSNASASSSSNEGDSDRDEKKRKQLKKAKMAKDRKSRKKPVEVKKGKDPNAPKRPMSAYMLWLNASREKIKSDHPGISITDLSKKAGEIWKGMSKEKKEEWDRKAEDARRDYEKAMKEYEGGRGESSKRDKSKKKKKVKVKMEKKSTPSRGSSSKSSSRQLSESFKSKEFVSSDESSSGENKSKKKRRRSEDSEEEELASTPPSSEDSASGSDE

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 structure specific recognition protein 1
别名 3D3H4, Chromatin-specific transcription elongation factor 80 kDa subunit, Facilitates chromatin transcription complex 80 kDa subunit, Facilitates chromatin transcription complex subunit SSRP1, FACT
计算分子量 81 kDa
观测分子量 86 kDa
GenBank蛋白编号BC005116
基因名称 SSRP1
Gene ID (NCBI) 6749
RRIDAB_2919701
偶联类型 CoraLite®555 Fluorescent Dye
最大激发/发射波长557 nm / 570 nm
激发激光Green Laser (532 nm), Yellow-Green Laser (561 nm)
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ08945
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL555 SSRP1 antibody CL555-67313Download protocol
IF protocol for CL555 SSRP1 antibody CL555-67313Download protocol
Standard Protocols
Click here to view our Standard Protocols
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