Various lysates were subjected to SDS PAGE followed by western blot with 68094-1-Ig (SPTLC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68094-1-PBS in a different storage buffer formulation.

WB result of SPTLC2 antibody (68094-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SPTLC2 transfected HEK-293 cells. This data was developed using the same antibody clone with 68094-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 68094-1-Ig (SPTLC2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68094-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68094-1-Ig (SPTLC2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68094-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 68094-1-Ig (SPTLC2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68094-1-PBS in a different storage buffer formulation.