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SPTLC2 Monoclonal antibody

SPTLC2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2G6A12

Cat No : 68094-1-Ig

Print datasheet

Synonyms

KIAA0526, LCB 2, LCB2, LCB2a, Serine palmitoyltransferase 2, SPT 2, SPT2, SPTLC2

验证数据展示

  • WB analysis using 68094-1-Ig

    WB analysis using 68094-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68094-1-Ig (SPTLC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • WB analysis of HEK-293 using 68094-1-Ig

    WB analysis of HEK-293 using 68094-1-Ig

    WB result of SPTLC2 antibody (68094-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SPTLC2 transfected HEK-293 cells.

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, HepG2 cells, A549 cells, K-562 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68094-1-Ig targets SPTLC2 in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA
Tested Reactivity Human
Immunogen SPTLC2 fusion protein Ag28380 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name serine palmitoyltransferase, long chain base subunit 2
Synonyms KIAA0526, LCB 2, LCB2, LCB2a, Serine palmitoyltransferase 2, SPT 2, SPT2, SPTLC2
Calculated Molecular Weight 63 kDa
Observed Molecular Weight60 kDa
GenBank Accession NumberBC005123
Gene Symbol SPTLC2
Gene ID (NCBI) 9517
RRIDAB_2918831
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO15270
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Serine palmitoyltransferase long chain base subunit 2 (SPTLC2) is one of the subunits of the heterodimeric molecule Serine palmitoyltransferase (SPT). Sptlc2 deficiency not only affected T cell sphingolipid anabolism but also led to prolonged activation of the mechanistic target of rapamycin complex 1 (PMID: 30952607).

实验方案

Product Specific Protocols
WB protocol for SPTLC2 antibody 68094-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols