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验证数据展示

  • WB analysis using 68094-1-Ig

    WB analysis using 68094-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68094-1-Ig (SPTLC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • WB analysis of HEK-293 using 68094-1-Ig

    WB analysis of HEK-293 using 68094-1-Ig

    WB result of SPTLC2 antibody (68094-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SPTLC2 transfected HEK-293 cells.

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  • KD/KO验证

SPTLC2 Monoclonal antibody

SPTLC2 Monoclonal Antibody for WB, ELISA
Cat No. 68094-1-Ig

产品说明书

CloneNo. 2G6A12

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, ELISA

别名

KIAA0526, LCB 2, LCB2, LCB2a, Serine palmitoyltransferase 2, SPT 2, SPT2, SPTLC2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, HepG2 cells, A549 cells, K-562 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68094-1-Ig targets SPTLC2 in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原

CatNo: Ag28380

Product name: Recombinant human SPTLC2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 254-562 aa of BC005123

Sequence: CLILSDELNHASLVLGARLSGATIRIFKHNNMQSLEKLLKDAIVYGQPRTRRPWKKILILVEGIYSMEGSIVRLPEVIALKKKYKAYLYLDEAHSIGALGPTGRGVVEYFGLDPEDVDVMMGTFTKSFGASGGYIGGKKELIDYLRTHSHSAVYATSLSPPVVEQIITSMKCIMGQDGTSLGKECVQQLAENTRYFRRRLKEMGFIIYGNEDSPVVPLMLYMPAKIGAFGREMLKRNIGVVVVGFPATPIIESRARFCLSAAHTKEILDTALKEIDEVGDLLQLKYSRHRLVPLLDRPFDETTYEETED

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 serine palmitoyltransferase, long chain base subunit 2
别名 KIAA0526, LCB 2, LCB2, LCB2a, Serine palmitoyltransferase 2, SPT 2, SPT2, SPTLC2
计算分子量 63 kDa
观测分子量60 kDa
GenBank蛋白编号BC005123
基因名称 SPTLC2
Gene ID (NCBI) 9517
RRIDAB_2918831
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDO15270
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Serine palmitoyltransferase long chain base subunit 2 (SPTLC2) is one of the subunits of the heterodimeric molecule Serine palmitoyltransferase (SPT). Sptlc2 deficiency not only affected T cell sphingolipid anabolism but also led to prolonged activation of the mechanistic target of rapamycin complex 1 (PMID: 30952607).

实验方案

Product Specific Protocols
WB protocol for SPTLC2 antibody 68094-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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