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验证数据展示

  • WB analysis using 27801-1-AP (same clone as 27801-1-PBS)

    WB analysis using 27801-1-AP (same clone as 27801-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27801-1-AP (SPG7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27801-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 27801-1-AP (same clone as 27801-1-PBS)

    IP experiment of HeLa using 27801-1-AP (same clone as 27801-1-PBS)

    IP result of anti-SPG7 (IP:27801-1-AP, 4ug; Detection:27801-1-AP 1:500) with HeLa cells lysate 2800 ug. This data was developed using the same antibody clone with 27801-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 27801-1-AP (same clone as 27801-1-PBS)

    IHC staining of human liver using 27801-1-AP (same clone as 27801-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27801-1-AP (SPG7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27801-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 27801-1-AP (same clone as 27801-1-PBS)

    IHC staining of human liver using 27801-1-AP (same clone as 27801-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27801-1-AP (SPG7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27801-1-PBS in a different storage buffer formulation.

SPG7 Polyclonal antibody, PBS Only

SPG7 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 27801-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IP, Indirect ELISA

别名

CAR, CMAR, EC:3.4.24.-, Mitochondrial inner membrane m-AAA protease component paraplegin, Paraplegin

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

27801-1-PBS targets SPG7 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag26265

Product name: Recombinant human SPG7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-45 aa of BC007692

Sequence: VATEAQVPFLAMAGPEFVEVIGGLGAARVRSLFKEARARAPCIVY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 spastic paraplegia 7 (pure and complicated autosomal recessive)
别名 CAR, CMAR, EC:3.4.24.-, Mitochondrial inner membrane m-AAA protease component paraplegin, Paraplegin
计算分子量 88 kDa
观测分子量54 kDa, 70 kDa, 80kDa
GenBank蛋白编号BC007692
基因名称 SPG7
Gene ID (NCBI) 6687
RRIDAB_2918133
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UQ90
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SPG7 encodes a nuclear-encoded mitochondrial metalloprotease protein that is a member of the AAA protein family. Two transcript variants encoding distinct isoforms have been identified. The molecular weight of SPG7 is about 88-100 kDa (precursor),80 kDa (intermediate) and 70 kDa (mature)(PMID: 24767997), while the molecular weight of isoform 2 is about 54 kDa.

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