验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, LNCaP cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
27801-1-AP targets SPG7 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag26265 Product name: Recombinant human SPG7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-45 aa of BC007692 Sequence: VATEAQVPFLAMAGPEFVEVIGGLGAARVRSLFKEARARAPCIVY 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | spastic paraplegia 7 (pure and complicated autosomal recessive) |
| 别名 | CAR, CMAR, EC:3.4.24.-, Mitochondrial inner membrane m-AAA protease component paraplegin, Paraplegin |
| 计算分子量 | 88 kDa |
| 观测分子量 | 54 kDa, 70 kDa, 80kDa |
| GenBank蛋白编号 | BC007692 |
| 基因名称 | SPG7 |
| Gene ID (NCBI) | 6687 |
| RRID | AB_2918133 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UQ90 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SPG7 encodes a nuclear-encoded mitochondrial metalloprotease protein that is a member of the AAA protein family. Two transcript variants encoding distinct isoforms have been identified. The molecular weight of SPG7 is about 88-100 kDa (precursor),80 kDa (intermediate) and 70 kDa (mature)(PMID: 24767997), while the molecular weight of isoform 2 is about 54 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for SPG7 antibody 27801-1-AP | Download protocol |
| IP protocol for SPG7 antibody 27801-1-AP | Download protocol |
| WB protocol for SPG7 antibody 27801-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
