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验证数据展示

  • IF Staining of A431 using CL488-84012-5

    IF Staining of A431 using CL488-84012-5

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 SP100 antibody (CL488-84012-5, Clone: 241153F8 ) at dilution of 1:200, Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

CoraLite® Plus 488-conjugated SP100 Recombinant antibody

SP100 Recombinant Antibody for IF/ICC
Cat No. CL488-84012-5

产品说明书

CloneNo. 241153F8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC

别名

241153F8, Nuclear autoantigen Sp-100, Nuclear dot-associated Sp100 protein, SP 100, SP100 nuclear antigen

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy SP100 Ready-To-Use IHC Kit. SP100 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 84012-5-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-84012-5 targets SP100 in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原 SP100 fusion protein Ag35193 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 SP100 nuclear antigen
别名 241153F8, Nuclear autoantigen Sp-100, Nuclear dot-associated Sp100 protein, SP 100, SP100 nuclear antigen
计算分子量 879 aa, 100 kDa
观测分子量70-100 kDa, 110-140 kDa
GenBank蛋白编号BC011562
基因名称 SP100
Gene ID (NCBI) 6672
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDP23497
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SP100 nuclear antigen, belongs to the nuclear bodies(NBs), has a role in the control of gene expression. It contains a HSR domain, which is important for the nuclear body targeting as well as for the dimerization, and one PxVxL motif, which if required for interaction with chromoshadow domains. Sp100 is an acidic protein that exerts a highly aberrant electrophoretic mobility in SDS-polyacrylamide gel electrophoresis.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 SP100 antibody CL488-84012-5Download protocol
Standard Protocols
Click here to view our Standard Protocols