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验证数据展示

  • WB analysis using 84012-5-RR

    WB analysis using 84012-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84012-5-RR (SP100 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 84012-5-RR

    IHC staining of human ovary cancer using 84012-5-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84012-5-RR (SP100 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with .

    Ready-to-use IHC kits available with SP100 antibody included
  • IHC staining of human ovary cancer using 84012-5-RR

    IHC staining of human ovary cancer using 84012-5-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84012-5-RR (SP100 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with .

    Ready-to-use IHC kits available with SP100 antibody included
  • IF Staining of A431 using 84012-5-RR

    IF Staining of A431 using 84012-5-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using SP100 antibody (84012-5-RR, Clone: 241153F8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of A431 using 84012-5-RR

    IF Staining of A431 using 84012-5-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using SP100 antibody (84012-5-RR, Clone: 241153F8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

SP100 Recombinant antibody

SP100 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 84012-5-RR

产品说明书

CloneNo. 241153F8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

241153F8, Nuclear autoantigen Sp-100, Nuclear dot-associated Sp100 protein, SP 100, SP100 nuclear antigen

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, HeLa cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy SP100 Ready-To-Use IHC Kit. SP100 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84012-5-RR targets SP100 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 SP100 fusion protein Ag35193 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 SP100 nuclear antigen
别名 241153F8, Nuclear autoantigen Sp-100, Nuclear dot-associated Sp100 protein, SP 100, SP100 nuclear antigen
计算分子量 879 aa, 100 kDa
观测分子量70-100 kDa, 110-140 kDa
GenBank蛋白编号BC011562
基因名称 SP100
Gene ID (NCBI) 6672
RRIDAB_3671580
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP23497
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SP100 nuclear antigen, belongs to the nuclear bodies(NBs), has a role in the control of gene expression. It contains a HSR domain, which is important for the nuclear body targeting as well as for the dimerization, and one PxVxL motif, which if required for interaction with chromoshadow domains. Sp100 is an acidic protein that exerts a highly aberrant electrophoretic mobility in SDS-polyacrylamide gel electrophoresis.

实验方案

Product Specific Protocols
WB protocol for SP100 antibody 84012-5-RRDownload protocol
IHC protocol for SP100 antibody 84012-5-RRDownload protocol
IF protocol for SP100 antibody 84012-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols