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验证数据展示

  • FC experiment of C6 using CL488-66786

    FC experiment of C6 using CL488-66786

    1X10^6 C6 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human SOX10 (CL488-66786, Clone:1D2C8) (red), or 0.4 ug CoraLite® Plus 488 Mouse IgG2a Isotype Control (C1.18.4) (CL488-65208, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IF Staining of mouse brain using CL488-66786

    IF Staining of mouse brain using CL488-66786

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 SOX10 antibody (CL488-66786, Clone: 1D2C8 ) at dilution of 1:200.

  • IF Staining of rat brain using CL488-66786

    IF Staining of rat brain using CL488-66786

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using CoraLite® Plus 488 SOX10 antibody (CL488-66786, Clone: 1D2C8 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated SOX10 Monoclonal antibody

SOX10 Monoclonal Antibody for IF-P, FC (Intra)
Cat No. CL488-66786

产品说明书

CloneNo. 1D2C8

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

IF-P, FC (Intra)

别名

Transcription factor SOX-10, Transcription factor SOX 10, SRY-box transcription factor 9, PCWH, DOM

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF-P detected inmouse brain tissue, rat brain tissue
Positive FC (Intra) detected inC6 cells
Planning an IHC experiment? We recommend our IHCeasy SOX10 Ready-To-Use IHC Kit. SOX10 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66786-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66786 targets SOX10 in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF-P, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag23191

Product name: Recombinant human SOX10 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 267-466 aa of BC002824

Sequence: GKPHIDFGNVDIGEISHEVMSNMETFDVAELDQYLPPNGHPGHVSSYSAAGYGLGSALAVASGHSAWISKPPGVALPTVSPPGVDAKAQVKTETAGPQGPPHYTDQPSTSQIAYTSLSLPHYGSAFPSISRPQFDYSDHQPSGPYYGHSGQASGLYSAFSYMGPSQRPLYTAISDPSPSGPQSHSPTHWEQPVYTTLSRP

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 SRY (sex determining region Y)-box 10
别名 Transcription factor SOX-10, Transcription factor SOX 10, SRY-box transcription factor 9, PCWH, DOM
计算分子量 50 kDa
GenBank蛋白编号BC002824
基因名称 SOX10
Gene ID (NCBI) 6663
RRIDAB_2919369
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDP56693
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sox10 is a transcription factor that has a crucial role in complex signalling pathways regulating central nervous system and neural crest development. The calcualted molecular weight of SOX10 is 50 kDa, but modified SOX10 is about 55-60 kDa. (PMID: 23799842)

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 SOX10 antibody CL488-66786Download protocol
IF protocol for CL Plus 488 SOX10 antibody CL488-66786Download protocol
Standard Protocols
Click here to view our Standard Protocols
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