验证数据展示
经过测试的应用
| Positive WB detected in | A375 cells, MDA-MB-453 cells |
| Positive IHC-Autostainer detected in | human appendicitis tissue, human skin cancer tissue, human malignant melanoma tissue, human breast tissue |
| Positive IHC detected in | human malignant melanoma tissue, human pituitary adenoma tissue, mouse brain tissue, rat cerebellum tissue, human breast tissue, human skin cancer tissue, human appendicitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | rat brain tissue, mouse brain tissue |
| Positive IF-Fro detected in | mouse brain tissue |
| Positive FC (Intra) detected in | C6 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC)-AUTOSTAINER | IHC-AUTOSTAINER : 1:5000-1:20000 |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66786-1-Ig targets SOX10 in WB, IHC, IHC-Autostainer, IF-P, IF-Fro, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IHC-Autostainer, IF-P, IF-Fro, FC (Intra), ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, rabbit, chicken, zebrafish |
| 免疫原 |
CatNo: Ag23191 Product name: Recombinant human SOX10 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 267-466 aa of BC002824 Sequence: GKPHIDFGNVDIGEISHEVMSNMETFDVAELDQYLPPNGHPGHVSSYSAAGYGLGSALAVASGHSAWISKPPGVALPTVSPPGVDAKAQVKTETAGPQGPPHYTDQPSTSQIAYTSLSLPHYGSAFPSISRPQFDYSDHQPSGPYYGHSGQASGLYSAFSYMGPSQRPLYTAISDPSPSGPQSHSPTHWEQPVYTTLSRP 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | SRY (sex determining region Y)-box 10 |
| 别名 | 1D2C8, DOM, PCWH, SRY-box transcription factor 9, Transcription factor SOX 10 |
| 计算分子量 | 50 kDa |
| 观测分子量 | 55-60 kDa |
| GenBank蛋白编号 | BC002824 |
| 基因名称 | SOX10 |
| Gene ID (NCBI) | 6663 |
| RRID | AB_2882131 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P56693 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Sox10 is a transcription factor that has a crucial role in complex signalling pathways regulating central nervous system and neural crest development. The calcualted molecular weight of SOX10 is 50 kDa, but modified SOX10 is about 55-60 kDa. (PMID: 23799842).SOX10 sumoylation by UBC9 (sumo-conjugating enzyme UBC9) is the best described one. Occurring at lysines K55, K246 and K357,19 it inhibits NC development and promotes development of non-sensory cranial placodes in vivo.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for SOX10 antibody 66786-1-Ig | Download protocol |
| IF protocol for SOX10 antibody 66786-1-Ig | Download protocol |
| IHC protocol for SOX10 antibody 66786-1-Ig | Download protocol |
| WB protocol for SOX10 antibody 66786-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Brown adipose tissue secretes OLFM4 to coordinate sensory and sympathetic innervation via Schwann cells | ||
Nucleic Acids Res SRSF10 regulates oligodendrocyte differentiation during mouse central nervous system development by modulating pre-mRNA splicing | ||
J Neuroinflammation SIRT6 modulates lesion microenvironment in LPC induced demyelination by targeting astrocytic CHI3L1 |
