CoraLite®594-conjugated SOD2 Monoclonal antibody
SOD2 Monoclonal Antibody for FC (Intra), IF/ICC
Host / Isotype
Mouse / IgG2a
Reactivity
Human, mouse, rat, pig and More (1)
Applications
FC (Intra), IF/ICC and More (1)
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
3A6C2
验证数据展示
经过测试的应用
| Positive IF detected in | HUVEC cells |
| Positive FC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy SOD2 Ready-To-Use IHC Kit. SOD2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66474-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.50 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
| FC | See 1 publications below |
产品信息
CL594-66474 targets SOD2 in IF, FC, FC (Intra) applications and shows reactivity with Human, mouse, rat, pig samples.
| Tested Applications | FC (Intra), IF/ICC |
| Cited Applications | IF, FC |
| Tested Reactivity | Human, mouse, rat, pig |
| Cited Reactivity | human |
| Immunogen | SOD2 fusion protein Ag21388 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | superoxide dismutase 2, mitochondrial |
| Synonyms | IPO B, Mn SOD, MNSOD, SOD2, SODM |
| Calculated Molecular Weight | 25 kDa |
| Observed Molecular Weight | 25 kDa |
| GenBank Accession Number | BC016934 |
| Gene Symbol | SOD2 |
| Gene ID (NCBI) | 6648 |
| RRID | AB_2883586 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P04179 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SOD2(superoxide dismutase 2, mitochondrial ) is also named as IPOB, MNSOD, SODM, Mn-SOD and belongs to the iron/manganese superoxide dismutase family. It is a marker of mitochondria, which is restricted to the perinuclear area in a cell with aggregate formation of mutant SOD1(PMID:12659845). It functions as the first line of antioxidant defense against highly reactive superoxide radicals and it appears to be early predictors for survival in septic patients with with MIF(PMID:20863520). It has 2 isoforms with the molecular weight of 25 kDa and 21 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 SOD2 antibody CL594-66474 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
EBioMedicine Single-nucleus RNA sequencing reveals heterogenous microenvironments and specific drug response between cervical squamous cell carcinoma and adenocarcinoma |
