• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • FC experiment of HeLa using CL555-66474

    FC experiment of HeLa using CL555-66474

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite®555 Anti-Human SOD2 (CL555-66474, Clone:3A6C2) (red), or 0.8 ug CoraLite® Plus 555 Mouse IgG2a Isotype Control (C1.18.4) (CL555-65208, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • IF Staining of A549 using CL555-66474

    IF Staining of A549 using CL555-66474

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CoraLite®555 SOD2 antibody (CL555-66474, Clone: 3A6C2 ) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite®555-conjugated SOD2 Monoclonal antibody

SOD2 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL555-66474

产品说明书

CloneNo. 3A6C2

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat, pig

应用

IF/ICC, FC (Intra)

别名

SODM, 3A6C2, EC:1.15.1.1, IPO B, IPOB

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite®555
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy SOD2 Ready-To-Use IHC Kit. SOD2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66474-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-66474 targets SOD2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag21388

Product name: Recombinant human SOD2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-140 aa of BC016934

Sequence: MLSRAVCGTSRQLAPALGYLGSRQKHSLPDLPYDYGALEPHINAQIMQLHHSKHHAAYVNNLNVTEEKYQEALAKGRFQAERREAVPGRGDPREPGPIRTGLSVEENSLRICTGSEFSRHDSLSFKHMVYLIVEGVPRWV

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 superoxide dismutase 2, mitochondrial
别名 SODM, 3A6C2, EC:1.15.1.1, IPO B, IPOB
计算分子量 25 kDa
观测分子量 25 kDa
GenBank蛋白编号BC016934
基因名称 SOD2
Gene ID (NCBI) 6648
RRIDAB_2919686
偶联类型 CoraLite®555 Fluorescent Dye
最大激发/发射波长557 nm / 570 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDP04179
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SOD2(superoxide dismutase 2, mitochondrial ) is also named as IPOB, MNSOD, SODM, Mn-SOD and belongs to the iron/manganese superoxide dismutase family. It is a marker of mitochondria, which is restricted to the perinuclear area in a cell with aggregate formation of mutant SOD1(PMID:12659845). It functions as the first line of antioxidant defense against highly reactive superoxide radicals and it appears to be early predictors for survival in septic patients with with MIF(PMID:20863520). It has 2 isoforms with the molecular weight of 25 kDa and 21 kDa.

实验方案

Product Specific Protocols
IF protocol for CL555 SOD2 antibody CL555-66474Download protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat Able
正在加载，请稍候...