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CoraLite® Plus 488-conjugated SNX9 Monoclonal antibody

SNX9 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B11C9

Cat No : CL488-68255

Print datasheet

Synonyms

MST155, MSTP155, Protein SDP1, SDP1, SH3PX1, SH3PXD3A, SNX9, sorting nexin 9, WISP

验证数据展示

  • IF Staining of A431 using CL488-68255

    IF Staining of A431 using CL488-68255

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using CoraLite® Plus 488 SNX9 antibody (CL488-68255, Clone: 1B11C9 ) at dilution of 1:200.

经过测试的应用

Positive IF detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 68255-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68255 targets SNX9 in IF applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF/ICC
Tested Reactivity Human, mouse, rat
Immunogen SNX9 fusion protein Ag8446 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name sorting nexin 9
Synonyms MST155, MSTP155, Protein SDP1, SDP1, SH3PX1, SH3PXD3A, SNX9, sorting nexin 9, WISP
Calculated Molecular Weight 595 aa, 67 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC005022
Gene Symbol SNX9
Gene ID (NCBI) 51429
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y5X1
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sorting nexins are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). They are involved in endocytosis and protein trafficking. SNX9 (Sorting nexin-9, also known as SH3PX1) was originally identified as a protein that interacted with the metalloproteinases ADAM9 and ADAM15. It contains a PX and an Sec homology 3 (SH3) domain. SNX9 functions in clathrin-mediated endocytosis and clathrin-independent, actin-dependent fluid-phase endocytosis (PMID: 17609109). On SDS-PAGE, SNX9 migrates at a molecular weight (70-78 kDa) higher than its calculated molecular mass of 67 kDa (PMID: 15299020; 12952949; 18411244; 15703209).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 SNX9 antibody CL488-68255Download protocol
Standard Protocols
Click here to view our Standard Protocols