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验证数据展示

  • WB analysis using 82755-3-RR

    WB analysis using 82755-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82755-3-RR (SNAI2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 82755-3-RR

    IF Staining of A431 using 82755-3-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using SNAI2 antibody (82755-3-RR, Clone: 250290A2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

SNAI2/SLUG Recombinant antibody

SNAI2/SLUG Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 82755-3-RR

产品说明书

CloneNo. 250290A2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

SNAI2, Protein snail homolog 2, SLUG, SLUGH, SLUGH1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse spleen tissue, rat spleen tissue, rat testis tissue
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82755-3-RR targets SNAI2/SLUG in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SNAI2/SLUG fusion protein Ag2773 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 snail homolog 2 (Drosophila)
别名 SNAI2, Protein snail homolog 2, SLUG, SLUGH, SLUGH1
计算分子量 268 aa, 30 kDa
观测分子量30-35 kDa
GenBank蛋白编号BC015895
基因名称 SLUG
Gene ID (NCBI) 6591
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO43623
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SNAI2 belongs to the Snail family of zinc finger transcription factors, which shares an evolutionarily conserved role in mesoderm formation in vertebrates and invertebrates. It tiggers epithelial-mesenchymal transitions and has a crucial role in developmental processes. Also it is a transcriptional reperssor in mediating RAF-induced expression of TJ protein, occludin(OCLN) and subsequent oncogenic transformation of epithelial cells.

实验方案

Product Specific Protocols
WB protocol for SNAI2/SLUG antibody 82755-3-RRDownload protocol
IF protocol for SNAI2/SLUG antibody 82755-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols