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验证数据展示

  • WB analysis using 82755-3-RR (same clone as 82755-3-PBS)

    WB analysis using 82755-3-RR (same clone as 82755-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82755-3-RR (SNAI2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82755-3-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 82755-3-RR (same clone as 82755-3-PBS)

    IF Staining of A431 using 82755-3-RR (same clone as 82755-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using SNAI2 antibody (82755-3-RR, Clone: 250290A2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82755-3-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 82755-3-RR (same clone as 82755-3-PBS)

    FC experiment of MCF-7 using 82755-3-RR (same clone as 82755-3-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug SNAI2/SLUG Recombinant monoclonal antibody (82755-3-RR, Clone:250290A2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82755-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82755-3-RR

    Affinity and Kinetic Characterization of 82755-3-RR

    Biolayer interferometry (BLl) kinetic assays of 82755-3-RR against Human SNAI2/SLUG were performed. The affinity constant is below 1 pM.

SNAI2/SLUG Recombinant monoclonal antibody, PBS Only

SNAI2/SLUG Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 82755-3-PBS

产品说明书

CloneNo. 250290A2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, FC (Intra), Indirect ELISA

别名

SNAI2, Protein snail homolog 2, SLUG, SLUGH, SLUGH1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

82755-3-PBS targets SNAI2/SLUG in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2773

Product name: Recombinant human SNAI2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-268 aa of BC015895

Sequence: MPRSFLVKKHFNASKKPNYSELDTHTVIISPYLYESYSMPVIPQPEILSSGAYSPITVWTTAAPFHAQLPNGLSPLSGYSSSLGRVSPPPPSDTSSKDHSGSESPISDEEERLQSKLSDPHAIEAEKFQCNLCNKTYSTFSGLAKHKQLHCDAQSRKSFSCKYCDKEYVSLGALKMHIRTHTLPCVCKICGKAFSRPWLLQGHIRTHTGEKPFSCPHCNRAFADRSNLRAHLQTHSDVKKYQCKNCSKTFSRMSLLHKHEESGCCVAH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 snail homolog 2 (Drosophila)
别名 SNAI2, Protein snail homolog 2, SLUG, SLUGH, SLUGH1
计算分子量 268 aa, 30 kDa
观测分子量30-35 kDa
GenBank蛋白编号BC015895
基因名称 SLUG
Gene ID (NCBI) 6591
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO43623
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SNAI2 belongs to the Snail family of zinc finger transcription factors, which shares an evolutionarily conserved role in mesoderm formation in vertebrates and invertebrates. It tiggers epithelial-mesenchymal transitions and has a crucial role in developmental processes. Also it is a transcriptional reperssor in mediating RAF-induced expression of TJ protein, occludin(OCLN) and subsequent oncogenic transformation of epithelial cells.

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