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验证数据展示

  • WB analysis using 18038-1-AP (same clone as 18038-1-PBS)

    WB analysis using 18038-1-AP (same clone as 18038-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18038-1-AP (SMURF2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18038-1-PBS in a different storage buffer formulation.

  • WB analysis using 18038-1-AP (same clone as 18038-1-PBS)

    WB analysis using 18038-1-AP (same clone as 18038-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18038-1-AP (SMURF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18038-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse testis using 18038-1-AP (same clone as 18038-1-PBS)

    IP experiment of mouse testis using 18038-1-AP (same clone as 18038-1-PBS)

    IP result of anti-SMURF2 (IP:18038-1-AP, 4ug; Detection:18038-1-AP 1:300) with mouse testis tissue lysate 4000 ug. This data was developed using the same antibody clone with 18038-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 18038-1-AP (same clone as 18038-1-PBS)

    IHC staining of human lung cancer using 18038-1-AP (same clone as 18038-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18038-1-AP (SMURF2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18038-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SMURF2 antibody included
  • Featured Product
  • KD/KO Validated

SMURF2 Polyclonal antibody, PBS Only

SMURF2 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 18038-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy SMURF2 Ready-To-Use IHC Kit. SMURF2 primary antibody included.

产品信息

18038-1-PBS targets SMURF2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag12666

Product name: Recombinant human SMURF2 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 390-748 aa of BC093876

Sequence: AGHCRIEVSREEIFEESYRQVMKMRPKDLWKRLMIKFRGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSRDDIYTLQINPDSAVNPEHLSYFHFVGRIMGMAVFHGHYIDGGFTLPFYKQLLGKSITLDDMELVDPDLHNSLVWILENDITGVLDHTFCVEHNAYGEIIQHELKPNGKSIPVNEENKKEYVRLYVNWRFLRGIEAQFLALQKGFNEVIPQHLLKTFDEKELELIICGLGKIDVNDWKVNTRLKHCTPDSNIVKWFWKAVEFFDEERRARLLQFVTGSSRVPLQGFKALQGAAGPRLFTIHQIDACTNNLPKAHTCFNRIDIPPYESYEKLYEKLLTAIEETCGFAVE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SMAD specific E3 ubiquitin protein ligase 2
别名
计算分子量 748 aa, 86 kDa
观测分子量86-100 kDa
GenBank蛋白编号BC093876
基因名称 SMURF2
Gene ID (NCBI) 64750
RRIDAB_3085549
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HAU4
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SMURF2 is a HECT domain E3 ubiquitin ligase involved in degradation of SMADs,TGF-beta receptor,and other substrates.It also functions in regulation of neuronal and planar cell polarity, induction of senescence, and tumor suppression(PMID:22231558).SMURF2 associates constitutively with SMAD7 and it is nuclear, but binding to SMAD7 induces export and recruitment to activated TGFBR, where it causes degradation of receptors and of SMAD7 via proteasomal and lysosomal pathways(PMIF:11163210). SMURF2 can be detected 100 kDa by poly-sumoylated (PMID:26679521).

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