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验证数据展示

  • WB analysis using 83771-5-RR

    WB analysis using 83771-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83771-5-RR (SMUG1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of U-2 OS using 83771-5-RR

    FC experiment of U-2 OS using 83771-5-RR

    1x10^6 U-2 OS were intracellularly stained with 0.25 ug SMUG1 Recombinant antibody (83771-5-RR, Clone:240817E4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 83771-5-RR

    Affinity and Kinetic Characterization of 83771-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83771-5-RR against Human SMUG1 were performed. The affinity constant is below 1 pM.

SMUG1 Recombinant monoclonal antibody

SMUG1 Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83771-5-RR

产品说明书

CloneNo. 240817E4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

UNG3, Single-strand selective monofunctional uracil DNA glycosylase, HMUDG, FDG, EC:3.2.2.-

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMOLT-4 cells, K-562 cells, HEK-293 cells, COLO 320 cells, HeLa cells
Positive FC (Intra) detected inU-2 OS

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83771-5-RR targets SMUG1 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag13934

Product name: Recombinant human SMUG1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-134 aa of BC000417

Sequence: MPQAFLLGSIHEPAGALMEPQPCPGSLAESFLEEELRLNAELSQLQFSEPVGIIYNPVEYAWEPHRNYVTRYCQGPKEVLFLGMNPGPFGMAQTGVPFGEVSMVRDWLGIVGPVLTPPQEHPKRPVLGLECPQS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 single-strand-selective monofunctional uracil-DNA glycosylase 1
别名 UNG3, Single-strand selective monofunctional uracil DNA glycosylase, HMUDG, FDG, EC:3.2.2.-
计算分子量 177 aa, 20 kDa
观测分子量30 kDa
GenBank蛋白编号BC000417
基因名称 SMUG1
Gene ID (NCBI) 23583
RRIDAB_3671365
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ53HV7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UNG(Uracil-DNA glycosylase) removes uracil in DNA resulting from deamination of cytosine or replicative incorporation of dUMP instead of dTMP. Thus, UNG plays a role in suppressing GC-to-AT transition mutations.The UNG gene encodes 2 isoforms that are individually targeted to the mitochondria and the nucleus(PMID:12369930).Defects in UNG are a cause of immunodeficiency with hyper-IgM type 5 (HIGM5).

实验方案

Product Specific Protocols
WB protocol for SMUG1 antibody 83771-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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