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SMU1 Polyclonal antibody

SMU1 Polyclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 15511-1-AP

Print datasheet

Synonyms

BWD, fSAP57, RP11 54K16.3, SMU 1, SMU1

验证数据展示

  • WB analysis of mouse liver using 15511-1-AP

    WB analysis of mouse liver using 15511-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 15511-1-AP (SMU1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 15511-1-AP

    IHC staining of human kidney using 15511-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 15511-1-AP (SMU1 antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with SMU1 antibody included
  • IHC staining of human kidney using 15511-1-AP

    IHC staining of human kidney using 15511-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 15511-1-AP (SMU1 antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with SMU1 antibody included
  • IF Staining of HepG2 using 15511-1-AP

    IF Staining of HepG2 using 15511-1-AP

    Immunofluorescent analysis of HepG2 cells, using SMU1 antibody 15511-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

经过测试的应用

Positive WB detected inmouse liver tissue, K-562 cells
Positive IHC detected inhuman kidney tissue, human brain tissue, human hepatocirrhosis tissue, human ovary tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy SMU1 Ready-To-Use IHC Kit. SMU1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15511-1-AP targets SMU1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, WB,ELISA
Tested Reactivity human, mouse, rat
Immunogen SMU1 fusion protein Ag7688 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name smu-1 suppressor of mec-8 and unc-52 homolog (C. elegans)
Synonyms BWD, fSAP57, RP11 54K16.3, SMU 1, SMU1
Calculated Molecular Weight 58 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC002876
Gene Symbol SMU1
Gene ID (NCBI) 55234
RRIDAB_2270843
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ2TAY7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sum1, belongs to the WD repeat SMU1 family. Human SMU1 is a component of the spliceosome fSAP57(PMID: 15254247). It is possible that the mutation in Smu1 affects spliceosome function, resulting in generation of improper splice variants, which could explain the phenotypes of tsTM18 cells. Some results suggest that Smu1 participates in at least two steps of splicing: splice-site choice and control of accuracy(PMID: 17105761). This antibody is a rabbit polyclonal antibody raised against a region of human REDD1 antigen.

实验方案

Product Specific Protocols
WB protocol for SMU1 antibody 15511-1-APDownload protocol
IHC protocol for SMU1 antibody 15511-1-APDownload protocol
IF protocol for SMU1 antibody 15511-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols