验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, HEK-293T cells, MOLT-4 cells, SH-SY5Y cells |
| Positive FC (Intra) detected in | HeLa cells, U2OS cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83520-3-RR targets SMPD2 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, FC (Intra), ELISA Application Description |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag7367 Product name: Recombinant human SMPD2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-301 aa of BC000038 Sequence: MKPNFSLRLRIFNLNCWGIPYLSKHRADRMRRLGDFLNQESFDLALLEEVWSEQDFQYLRQKLSPTYPAAHHFRSGIIGSGLCVFSKHPIQELTQHIYTLNGYPYMIHHGDWFSGKAVGLLVLHLSGMVLNAYVTHLHAEYNRQKDIYLAHRVAQAWELAQFIHHTSKKADVVLLCGDLNMHPEDLGCCLLKEWTGLHDAYLETRDFKGSEEGNTMVPKNCYVSQQELKPFPFGVRIDYVLYKAVSGFYISCKSFETTTGFDPHSGTPLSDHEALMATLFVRHSPPQQNPSSTHGPAERSP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | sphingomyelin phosphodiesterase 2, neutral membrane (neutral sphingomyelinase) |
| 别名 | 240400B5, EC:3.1.4.12, Lyso-PAF-PLC, Lyso-platelet-activating factor-phospholipase C, Neutral sphingomyelinase |
| 计算分子量 | 48 kDa |
| 观测分子量 | 48 kDa |
| GenBank蛋白编号 | BC000038 |
| 基因名称 | SMPD2 |
| Gene ID (NCBI) | 6610 |
| RRID | AB_3671146 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O60906 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SMPD2, Sphingomyelin phosphodiesterase 2, is also named as Lyso-platelet-activating factor-phospholipase C and neutral sphingomyelinase (nSMase). It is involved in the sphingolipid metabolism pathway, which can catalyze the hydrolysis of sphingomyelin to form ceramide and phosphocholine. Smpd2/Smpd3 double-mutant mice were found to have severe retardation of late embryonic and postnatal growth and delayed puberty, belonging to hypothalamus-induced combined pituitary hormone deficiency (PubMed: 15764706).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for SMPD2 antibody 83520-3-RR | Download protocol |
| WB protocol for SMPD2 antibody 83520-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
