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验证数据展示

  • WB analysis using 83520-3-RR (same clone as 83520-3-PBS)

    WB analysis using 83520-3-RR (same clone as 83520-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83520-3-RR (SMPD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83520-3-PBS in a different storage buffer formulation.

  • FC experiment of U2OS using 83520-3-RR (same clone as 83520-3-PBS)

    FC experiment of U2OS using 83520-3-RR (same clone as 83520-3-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug SMPD2 Recombinant antibody (83520-3-RR, Clone:240400B5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83520-3-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83520-3-RR (same clone as 83520-3-PBS)

    FC experiment of HeLa using 83520-3-RR (same clone as 83520-3-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug SMPD2 Recombinant antibody (83520-3-RR, Clone:240400B5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83520-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00518-1

    Cytometric bead array standard curve of MP00518-1

    Cytometric bead array standard curve of MP00518-1, SMPD2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83520-1-PBS. Detection antibody: 83520-3-PBS. Standard: Ag7367. Range: 0.78-100 ng/mL.

  • Affinity and Kinetic Characterization of 83520-3-RR

    Affinity and Kinetic Characterization of 83520-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83520-3-RR against Human SMPD2 were performed. The affinity constant is below 1 pM.

SMPD2 Recombinant monoclonal antibody, PBS Only (Detector)

SMPD2 Uni-rAb® Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 83520-3-PBS

产品说明书

CloneNo. 240400B5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), Cytometric bead array, Indirect ELISA

别名

240400B5, EC:3.1.4.12, Lyso-PAF-PLC, Lyso-platelet-activating factor-phospholipase C, Neutral sphingomyelinase

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83520-3-PBS targets SMPD2 as part of a matched antibody pair:

MP00518-1: 83520-1-PBS capture and 83520-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag7367

Product name: Recombinant human SMPD2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-301 aa of BC000038

Sequence: MKPNFSLRLRIFNLNCWGIPYLSKHRADRMRRLGDFLNQESFDLALLEEVWSEQDFQYLRQKLSPTYPAAHHFRSGIIGSGLCVFSKHPIQELTQHIYTLNGYPYMIHHGDWFSGKAVGLLVLHLSGMVLNAYVTHLHAEYNRQKDIYLAHRVAQAWELAQFIHHTSKKADVVLLCGDLNMHPEDLGCCLLKEWTGLHDAYLETRDFKGSEEGNTMVPKNCYVSQQELKPFPFGVRIDYVLYKAVSGFYISCKSFETTTGFDPHSGTPLSDHEALMATLFVRHSPPQQNPSSTHGPAERSP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 sphingomyelin phosphodiesterase 2, neutral membrane (neutral sphingomyelinase)
别名 240400B5, EC:3.1.4.12, Lyso-PAF-PLC, Lyso-platelet-activating factor-phospholipase C, Neutral sphingomyelinase
计算分子量 48 kDa
GenBank蛋白编号BC000038
基因名称 SMPD2
Gene ID (NCBI) 6610
RRIDAB_3671146
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO60906
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SMPD2, Sphingomyelin phosphodiesterase 2, is also named as Lyso-platelet-activating factor-phospholipase C and neutral sphingomyelinase (nSMase). It is involved in the sphingolipid metabolism pathway, which can catalyze the hydrolysis of sphingomyelin to form ceramide and phosphocholine. Smpd2/Smpd3 double-mutant mice were found to have severe retardation of late embryonic and postnatal growth and delayed puberty, belonging to hypothalamus-induced combined pituitary hormone deficiency (PubMed: 15764706).

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