验证数据展示
经过测试的应用
| Positive IF/ICC detected in | MDCK cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66851-2-Ig targets SMO in IF/ICC, ELISA applications and shows reactivity with Human, Canine samples.
| 经测试应用 | IF/ICC, ELISA Application Description |
| 经测试反应性 | Human, Canine |
| 免疫原 |
CatNo: Ag25861 Product name: Recombinant human SMO protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 28-233 aa of BC009989 Sequence: RGAASSGNATGPGPRSAGGSARRSAAVTGPPPPLSHCGRAAPCEPLRYNVCLGSVLPYGATSTLLAGDSDSQEEAHGKLVLWSGLRNAPRCWAVIQPLLCAVYMPKCENDRVELPSRTLCQATRGPCAIVERERGWPDFLRCTPDRFPEGCTNEVQNIKFNSSGQCEVPLVRTDNPKSWYEDVEGCGIQCQNPLFTEAEHQDMHSY 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | smoothened homolog (Drosophila) |
| 别名 | Gx, Protein Gx, SMO, SMOH, Smoothened homolog |
| 计算分子量 | 787 aa, 86 kDa |
| GenBank蛋白编号 | BC009989 |
| 基因名称 | SMO |
| Gene ID (NCBI) | 6608 |
| RRID | AB_3085033 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q99835 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Smoothened (SMO) is a G protein-coupled receptor that is a component of the hedgehog (Hh) signaling pathway and is conserved from flies to humans. The Hh pathway plays central roles in animal development and stem-cell function. Defects in Hh signalling lead to birth defects and cancer in humans. Expressed on the primary cilium, SMO interacts with the patched protein, a receptor for Hh proteins.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for SMO antibody 66851-2-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
