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验证数据展示

  • WB analysis using 12178-1-AP

    WB analysis using 12178-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12178-1-AP (SMNDC1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lymphoma using 12178-1-AP

    IHC staining of human lymphoma using 12178-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 12178-1-AP (SMNDC1 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human lymphoma using 12178-1-AP

    IHC staining of human lymphoma using 12178-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 12178-1-AP (SMNDC1 Antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of HepG2 using 12178-1-AP

    IF Staining of HepG2 using 12178-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMNDC1 antibody (12178-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

SMNDC1 Polyclonal antibody

SMNDC1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 12178-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Survival of motor neuron-related-splicing factor 30, Survival motor neuron domain-containing protein 1, SPF30, SMN-related protein, SMNR

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse heart tissue, human heart tissue, human lung tissue, human liver tissue, mouse skeletal muscle tissue, rat heart tissue
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12178-1-AP targets SMNDC1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2822

Product name: Recombinant human SMNDC1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-238 aa of BC011234

Sequence: MSEDLAKQLASYKAQLQQVEAALSGNGENEDLLKLKKDLQEVIELTKDLLSTQPSETLASSDSFASTQPTHSWKVGDKCMAVWSEDGQCYEAEIEEIDEENGTAAITFAGYGNAEVTPLLNLKPVEEGRKAKEDSGNKPMSKKEMIAQQREYKKKKALKKAQRIKELEQEREDQKVKWQQFNNRAYSKNKKGQVKRSIFASPESVTGKVGVGTCGIADKPMTQYQDTSKYNVRHLMPQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 survival motor neuron domain containing 1
别名 Survival of motor neuron-related-splicing factor 30, Survival motor neuron domain-containing protein 1, SPF30, SMN-related protein, SMNR
计算分子量 238 aa, 27 kDa
观测分子量 32 kDa
GenBank蛋白编号BC011234
基因名称 SMNDC1
Gene ID (NCBI) 10285
RRIDAB_2239665
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75940
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Survival motor neuron domain-containing protein 1 (SMNDC1), also known as survival of motor neuron-related-splicing factor 30 (SPF30) or SMN-related protein (SMNR), is a constituent of the spliceosome complex that plays an essential role in spliceosome assembly by recruiting U4/U5/U6 tri-snRNP to the pre-spliceosomal complex (PMID: 9817934; 11331595; 30904945). Both SMNDC1 and SMN1 contain a highly conserved central Tudor domain which functions as a protein-protein interaction surface and mediates binding to the spliceosomal Sm proteins (PMID: 11331595).

实验方案

Product Specific Protocols
WB protocol for SMNDC1 antibody 12178-1-APDownload protocol
IHC protocol for SMNDC1 antibody 12178-1-APDownload protocol
IF protocol for SMNDC1 antibody 12178-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols