SMNDC1 Polyclonal antibody

SMNDC1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 12178-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

Survival of motor neuron-related-splicing factor 30, Survival motor neuron domain-containing protein 1, SPF30, SMN-related protein, SMNR

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inmouse heart tissue, human heart tissue, human lung tissue, human liver tissue, mouse skeletal muscle tissue, rat heart tissue
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12178-1-AP targets SMNDC1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SMNDC1 fusion protein Ag2822 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 survival motor neuron domain containing 1
别名 Survival of motor neuron-related-splicing factor 30, Survival motor neuron domain-containing protein 1, SPF30, SMN-related protein, SMNR
计算分子量 238 aa, 27 kDa
观测分子量 32 kDa
GenBank蛋白编号BC011234
基因名称 SMNDC1
Gene ID (NCBI) 10285
RRIDAB_2239665
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75940
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Survival motor neuron domain-containing protein 1 (SMNDC1), also known as survival of motor neuron-related-splicing factor 30 (SPF30) or SMN-related protein (SMNR), is a constituent of the spliceosome complex that plays an essential role in spliceosome assembly by recruiting U4/U5/U6 tri-snRNP to the pre-spliceosomal complex (PMID: 9817934; 11331595; 30904945). Both SMNDC1 and SMN1 contain a highly conserved central Tudor domain which functions as a protein-protein interaction surface and mediates binding to the spliceosomal Sm proteins (PMID: 11331595).

实验方案

Product Specific Protocols
WB protocol for SMNDC1 antibody 12178-1-APDownload protocol
IHC protocol for SMNDC1 antibody 12178-1-APDownload protocol
IF protocol for SMNDC1 antibody 12178-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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