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验证数据展示

  • WB analysis using 14185-1-AP (same clone as 14185-1-PBS)

    WB analysis using 14185-1-AP (same clone as 14185-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14185-1-AP (SMC3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14185-1-PBS in a different storage buffer formulation.

  • WB analysis using 14185-1-AP (same clone as 14185-1-PBS)

    WB analysis using 14185-1-AP (same clone as 14185-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14185-1-AP (SMC3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14185-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach using 14185-1-AP (same clone as 14185-1-PBS)

    IHC staining of human stomach using 14185-1-AP (same clone as 14185-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 14185-1-AP (SMC3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14185-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 14185-1-AP (same clone as 14185-1-PBS)

    IF Staining of HeLa using 14185-1-AP (same clone as 14185-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SMC3 antibody (14185-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 14185-1-PBS in a different storage buffer formulation.

SMC3 Polyclonal antibody, PBS Only

SMC3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 14185-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

BAM, Bamacan, Basement membrane-associated chondroitin proteoglycan, BMH, CDLS3

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

14185-1-PBS targets SMC3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag5440

Product name: Recombinant human SMC3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-349 aa of BC047324

Sequence: MYIKQVIIQGFRSYRDQTIVDPFSSKHNVIVGRNGSGKSNLFYAIQFVLSDEFSHLRPEQRLALLHEGTGPRVISAFVEIIFDNSDNRLPIDKEEVSLRRVIGAKKDQYFLDKKMVTKNDVMNLLESAGFSRSNPYYIVKQGKINQMATAPDSQRLKLLREVAGTRVYDERKEESISLMKETEGKREKINELLKYIEERLHTLEEEKEELAQYQKWDKMRRALEYTIYNQELNETRAKLDELSAKRETSGEKSRQLRDAQQDARDKMEDIERQVRELKTKISAMKEEKEQLSAERQEQIKQRTKLELKAKDLQDELAGNSEQRKRLLKERQKLLEKIEEKQKELAETEP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 structural maintenance of chromosomes 3
别名 BAM, Bamacan, Basement membrane-associated chondroitin proteoglycan, BMH, CDLS3
计算分子量 142 kDa
观测分子量 140 kDa
GenBank蛋白编号BC047324
基因名称 SMC3
Gene ID (NCBI) 9126
RRIDAB_2192625
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UQE7
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
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