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SMC3 Polyclonal antibody

SMC3 Polyclonal Antibody for FC, IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, IF/ICC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 14185-1-AP

Print datasheet

Synonyms

BAM, Bamacan, BMH, CDLS3, CSPG6, HCAP, SMC 3, SMC protein 3, SMC3, SMC3L1

验证数据展示

  • WB analysis of HeLa using 14185-1-AP

    WB analysis of HeLa using 14185-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14185-1-AP (SMC3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 14185-1-AP

    FC experiment of HeLa using 14185-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SMC3 (14185-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HeLa using 14185-1-AP

    IF Staining of HeLa using 14185-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14185-1-AP (SMC3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, mouse brain tissue
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14185-1-AP targets SMC3 in WB, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF/ICC, WB, ELISA
Cited ApplicationsWB, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen SMC3 fusion protein Ag5440 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name structural maintenance of chromosomes 3
Synonyms BAM, Bamacan, BMH, CDLS3, CSPG6, HCAP, SMC 3, SMC protein 3, SMC3, SMC3L1
Calculated Molecular Weight 142 kDa
Observed Molecular Weight 140 kDa
GenBank Accession NumberBC047324
Gene Symbol SMC3
Gene ID (NCBI) 9126
RRIDAB_2192625
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UQE7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SMC3 antibody 14185-1-APDownload protocol
IF protocol for SMC3 antibody 14185-1-APDownload protocol
FC protocol for SMC3 antibody 14185-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanCoIP

Int J Mol Sci

APEX2-Mediated Proximity Labeling Resolves the DDIT4-Interacting Proteome.

Authors - Marianna Naki
View Article
humanWB

Biology (Basel)

In Situ Peroxidase Labeling Followed by Mass-Spectrometry Reveals TIA1 Interactome.

Authors - Olga Gourdomichali
View Article