验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, Jurkat cells, mouse brain tissue |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14185-1-AP targets SMC3 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag5440 Product name: Recombinant human SMC3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-349 aa of BC047324 Sequence: MYIKQVIIQGFRSYRDQTIVDPFSSKHNVIVGRNGSGKSNLFYAIQFVLSDEFSHLRPEQRLALLHEGTGPRVISAFVEIIFDNSDNRLPIDKEEVSLRRVIGAKKDQYFLDKKMVTKNDVMNLLESAGFSRSNPYYIVKQGKINQMATAPDSQRLKLLREVAGTRVYDERKEESISLMKETEGKREKINELLKYIEERLHTLEEEKEELAQYQKWDKMRRALEYTIYNQELNETRAKLDELSAKRETSGEKSRQLRDAQQDARDKMEDIERQVRELKTKISAMKEEKEQLSAERQEQIKQRTKLELKAKDLQDELAGNSEQRKRLLKERQKLLEKIEEKQKELAETEP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | structural maintenance of chromosomes 3 |
| 别名 | BAM, Bamacan, Basement membrane-associated chondroitin proteoglycan, BMH, CDLS3 |
| 计算分子量 | 142 kDa |
| 观测分子量 | 140 kDa |
| GenBank蛋白编号 | BC047324 |
| 基因名称 | SMC3 |
| Gene ID (NCBI) | 9126 |
| RRID | AB_2192625 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UQE7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for SMC3 antibody 14185-1-AP | Download protocol |
| IHC protocol for SMC3 antibody 14185-1-AP | Download protocol |
| WB protocol for SMC3 antibody 14185-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biology (Basel) In Situ Peroxidase Labeling Followed by Mass-Spectrometry Reveals TIA1 Interactome. | ||
Cell Mol Life Sci RetSat stabilizes mitotic chromosome segregation in pluripotent stem cells | ||
Heliyon ZNF521 promotes acute myeloid leukemogenesis by suppressing the expression and acetylation of SMC3 | ||
Curr Eye Res Cohesin Complex Interacting with Promoters of MMP Genes for in Pterygium Occurrence
| ||
Proc Natl Acad Sci U S A Gonadal sex and temperature independently influence germ cell differentiation and meiotic progression in Trachemys scripta |
