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验证数据展示

  • WB analysis of mouse brain using 10998-2-AP (same clone as 10998-2-PBS)

    WB analysis of mouse brain using 10998-2-AP (same clone as 10998-2-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10998-2-AP (SMARCD1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10998-2-PBS in a different storage buffer formulation.

  • IHC staining of human lymphoma using 10998-2-AP (same clone as 10998-2-PBS)

    IHC staining of human lymphoma using 10998-2-AP (same clone as 10998-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 10998-2-AP (SMARCD1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10998-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SMARCD1 antibody included
  • IF Staining of SH-SY5Y using 10998-2-AP (same clone as 10998-2-PBS)

    IF Staining of SH-SY5Y using 10998-2-AP (same clone as 10998-2-PBS)

    Immunofluorescent analysis of SH-SY5Y cells, using SMARCD1 antibody 10998-2-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 10998-2-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 10998-2-AP (same clone as 10998-2-PBS)

    IP experiment of mouse brain using 10998-2-AP (same clone as 10998-2-PBS)

    IP result of anti-SMARCD1 (IP:10998-2-AP, 3ug; Detection:10998-2-AP 1:600) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 10998-2-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

SMARCD1 Polyclonal antibody, PBS Only

SMARCD1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 10998-2-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy SMARCD1 Ready-To-Use IHC Kit. SMARCD1 primary antibody included.

产品信息

10998-2-PBS targets SMARCD1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1453

Product name: Recombinant human SMARCD1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 126-476 aa of BC009368

Sequence: RLDIQEALKRPIKQKRKLRIFISNTFNPAKSDAEDGEGTVASWELRVEGRLLEDSALSKYDATKQKRKFSSFFKSLVIELDKDLYGPDNHLVEWHRTATTQETDGFQVKRPGDVNVRCTVLLMLDYQPPQFKLDPRLARLLGIHTQTRPVIIQALWQYIKTHKLQDPHEREFVICDKYLQQIFESQRMKFSEIPQRLHALLMPPEPIIINHVISVDPNDQKKTACYDIDVEVDDTLKTQMNSFLLSTASQQEIATLDNKIHETIETINQLKTQREFMLSFARDPQGFINDWLQSQCRDLKTMTDVVGNPEEERRAEFYFQPWAQEAVCRYFYSKVQQRRQELEQALGIRNT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 1
别名
计算分子量 58 kDa
观测分子量 58 kDa
GenBank蛋白编号BC009368
基因名称 SMARCD1
Gene ID (NCBI) 6602
RRIDAB_2192133
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96GM5
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SMARCD1 belongs to the nuclear SWI/SNF chromatin remodeling complex, which regulates transcription and interacts with nucleolin in the nucleus. In addition, it's a member of the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). It possesses a SWIB domain which is homologous to and shares a common fold with the p53-binding domain of MDM2, a cell cycle regulator which interacts with NPM1. SMARCD1 has a strong influence on the Vitamin D-mediated transcriptional activity from an enhancer Vitamin D receptor element (VDRE). And it can mediates critical interactions between nuclear receptors and the BRG1/SMARCA4 chromatin-remodeling complex for transactivation. This antibody may have across reaction with SMARCD2.

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