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验证数据展示

  • WB analysis of mouse brain using 10998-2-AP

    WB analysis of mouse brain using 10998-2-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10998-2-AP (SMARCD1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human lymphoma using 10998-2-AP

    IHC staining of human lymphoma using 10998-2-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 10998-2-AP (SMARCD1 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with SMARCD1 antibody included
  • IF Staining of SH-SY5Y using 10998-2-AP

    IF Staining of SH-SY5Y using 10998-2-AP

    Immunofluorescent analysis of SH-SY5Y cells, using SMARCD1 antibody 10998-2-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • IP experiment of mouse brain using 10998-2-AP

    IP experiment of mouse brain using 10998-2-AP

    IP result of anti-SMARCD1 (IP:10998-2-AP, 3ug; Detection:10998-2-AP 1:600) with mouse brain tissue lysate 4000ug.

  • IHC staining of human stomach cancer using 10998-2-AP

    IHC staining of human stomach cancer using 10998-2-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10998-2-AP (SMARCD1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SMARCD1 antibody included
  • IHC staining of mouse brain using 10998-2-AP

    IHC staining of mouse brain using 10998-2-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10998-2-AP (SMARCD1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SMARCD1 antibody included
  • IHC staining of mouse stomach using 10998-2-AP

    IHC staining of mouse stomach using 10998-2-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 10998-2-AP (SMARCD1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SMARCD1 antibody included
  • IHC staining of rat brain using 10998-2-AP

    IHC staining of rat brain using 10998-2-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 10998-2-AP (SMARCD1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SMARCD1 antibody included
  • Featured Product
  • KD/KO Validated

SMARCD1 Polyclonal antibody

SMARCD1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 10998-2-AP

产品说明书

引用文献(11)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

别名

60 kDa BRG-1/Brm-associated factor subunit A, BAF60A, BRG1-associated factor 60A, CRACD1, Rsc6p

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, human brain tissue, Jurkat cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman lymphoma tissue, human stomach cancer tissue, rat brain tissue, mouse stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy SMARCD1 Ready-To-Use IHC Kit. SMARCD1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10998-2-AP targets SMARCD1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原

CatNo: Ag1453

Product name: Recombinant human SMARCD1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 126-476 aa of BC009368

Sequence: RLDIQEALKRPIKQKRKLRIFISNTFNPAKSDAEDGEGTVASWELRVEGRLLEDSALSKYDATKQKRKFSSFFKSLVIELDKDLYGPDNHLVEWHRTATTQETDGFQVKRPGDVNVRCTVLLMLDYQPPQFKLDPRLARLLGIHTQTRPVIIQALWQYIKTHKLQDPHEREFVICDKYLQQIFESQRMKFSEIPQRLHALLMPPEPIIINHVISVDPNDQKKTACYDIDVEVDDTLKTQMNSFLLSTASQQEIATLDNKIHETIETINQLKTQREFMLSFARDPQGFINDWLQSQCRDLKTMTDVVGNPEEERRAEFYFQPWAQEAVCRYFYSKVQQRRQELEQALGIRNT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 1
别名 60 kDa BRG-1/Brm-associated factor subunit A, BAF60A, BRG1-associated factor 60A, CRACD1, Rsc6p
计算分子量 58 kDa
观测分子量 58 kDa
GenBank蛋白编号BC009368
基因名称 SMARCD1
Gene ID (NCBI) 6602
RRIDAB_2192133
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96GM5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SMARCD1 belongs to the nuclear SWI/SNF chromatin remodeling complex, which regulates transcription and interacts with nucleolin in the nucleus. In addition, it's a member of the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). It possesses a SWIB domain which is homologous to and shares a common fold with the p53-binding domain of MDM2, a cell cycle regulator which interacts with NPM1. SMARCD1 has a strong influence on the Vitamin D-mediated transcriptional activity from an enhancer Vitamin D receptor element (VDRE). And it can mediates critical interactions between nuclear receptors and the BRG1/SMARCA4 chromatin-remodeling complex for transactivation. This antibody may have across reaction with SMARCD2.

实验方案

Product Specific Protocols
IF protocol for SMARCD1 antibody 10998-2-APDownload protocol
IHC protocol for SMARCD1 antibody 10998-2-APDownload protocol
IP protocol for SMARCD1 antibody 10998-2-APDownload protocol
WB protocol for SMARCD1 antibody 10998-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nat Commun

BAF60a deficiency uncouples chromatin accessibility and cold sensitivity from white fat browning.

Authors - Tongyu Liu
  • KO Validated
View Article
mouseCoIP

Nucleic Acids Res

HRP2-DPF3a-BAF complex coordinates histone modification and chromatin remodeling to regulate myogenic gene transcription.

Authors - Xu Zhu
View Article
humanWB

J Clin Invest

Genome-wide CRISPR screen identifies a cytokine-enhancer circuit driving HIF-2α activation in renal cancer.

Authors - Jun Fang
  • KO Validated
View Article
humanWB

Cancer Lett

MicroRNA-490-3P targets CDK1 and inhibits ovarian epithelial carcinoma tumorigenesis and progression.

Authors - Shuo Chen
View Article
humanWB

Biochem Pharmacol

ncBAF enhances PXR-mediated transcriptional activation in the human and mouse liver

Authors - Kiamu Kurosawa
View Article
humanWB

J Cell Mol Med

DLEU1 contributes to ovarian carcinoma tumourigenesis and development by interacting with miR-490-3p and altering CDK1 expression.

Authors - Li-Li Wang
View Article
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