IHCeasy SMARCD1 Ready-To-Use IHC Kit
SMARCD1 Ready-to-use reagent kit for IHC.
Reactivity
Human, Mouse, Rat
Sample Type
FFPE tissue
Cat no : KHC2794
Synonyms
60 kDa BRG-1/Brm-associated factor subunit A, BAF60A, BRG1-associated factor 60A, CRACD1, Rsc6p
Validation Data Gallery
Product Information
KHC2794 is a ready-to-use IHC kit for staining of SMARCD1. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.
| Product name | IHCeasy SMARCD1 Ready-To-Use IHC Kit |
| Sample type | FFPE tissue |
| Assay type | Immunohistochemistry |
| Primary antibody type | Rabbit Polyclonal |
| Secondary antibody type | Polymer-HRP-Goat anti-Rabbit |
| Reactivity | Human, Mouse, Rat |
Kit components
| Component | Size | Concentration |
|---|---|---|
| Antigen Retrieval Buffer | 100 mL | 50× |
| Washing Buffer | 100 mL ×2 | 20× |
| Blocking Buffer | 5 mL | RTU |
| Primary Antibody | 5 mL | RTU |
| Secondary Antibody | 5 mL | RTU |
| Chromogen Component A | 0.2 mL | RTU |
| Chromogen Component B | 4 mL | RTU |
| Signal Enhancer | 5 mL | RTU |
| Counter Staining Reagent | 5 mL | RTU |
| Mounting Media | 5 mL | RTU |
| Datasheet | 1 Copy | |
| Manual | 1 Copy |
Background Information
SMARCD1 belongs to the nuclear SWI/SNF chromatin remodeling complex, which regulates transcription and interacts with nucleolin in the nucleus. In addition, it's a member of the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). It possesses a SWIB domain which is homologous to and shares a common fold with the p53-binding domain of MDM2, a cell cycle regulator which interacts with NPM1. SMARCD1 has a strong influence on the Vitamin D-mediated transcriptional activity from an enhancer Vitamin D receptor element (VDRE). And it can mediates critical interactions between nuclear receptors and the BRG1/SMARCA4 chromatin-remodeling complex for transactivation.
Properties
| Storage Instructions | All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt. |
| Synonyms | 60 kDa BRG-1/Brm-associated factor subunit A, BAF60A, BRG1-associated factor 60A, CRACD1, Rsc6p |