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验证数据展示

  • WB analysis using 85019-3-RR

    WB analysis using 85019-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85019-3-RR (SMARCA5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 85019-3-RR

    FC experiment of HeLa using 85019-3-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug SMARCA5 Recombinant antibody (85019-3-RR, Clone:242512A8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IF Staining of HeLa using 85019-3-RR

    IF Staining of HeLa using 85019-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SMARCA5 antibody (85019-3-RR, Clone: 242512A8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

SMARCA5 Recombinant antibody

SMARCA5 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 85019-3-RR

产品说明书

CloneNo. 242512A8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), ELISA

别名

242512A8, EC:3.6.4.-, hISWI, hSNF2H, ISWI

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HCT 116 cells, HepG2 cells, MCF-7 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy SMARCA5 Ready-To-Use IHC Kit. SMARCA5 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85019-3-RR targets SMARCA5 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 SMARCA5 fusion protein Ag3759 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5
别名 242512A8, EC:3.6.4.-, hISWI, hSNF2H, ISWI
计算分子量 1052 aa, 122 kDa
观测分子量 135 kDa
GenBank蛋白编号BC023144
基因名称 SMARCA5
Gene ID (NCBI) 8467
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO60264
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SMARCA5, also named as SNF2H or WCRF135, is a 1052 amino acid protein, which localizes in nucleus and is overexpressed in CD34-positive erythrocyte progenitor cells in acute myeloid leukemia. SMARCA5 is one of the most conserved chromatin remodeling factors. It exists in a variety of oligosubunit complexes that move DNA with respect to the histone octamer to generate regularly spaced nucleosomal arrays. Smarca5 interacts with different accessory proteins and represents a molecular motor for DNA replication, repair, and transcription.

实验方案

Product Specific Protocols
WB protocol for SMARCA5 antibody 85019-3-RRDownload protocol
IF protocol for SMARCA5 antibody 85019-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols