验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy SLK Ready-To-Use IHC Kit. SLK primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 19743-1-AP
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-19743 targets SLK in IF/ICC applications and shows reactivity with human samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | STE20-like kinase (yeast) |
| 别名 | bA16H23.1, CTCL tumor antigen se20 9, hSLK, KIAA0204, LOSK, se20 9, SLK, STE20 like kinase, STE20 like kinase (yeast), STE20 related kinase, STK2 |
| 计算分子量 | 143 kDa |
| 观测分子量 | 143-160 kDa, 210-220 kDa |
| GenBank蛋白编号 | NM_014720 |
| 基因名称 | SLK |
| Gene ID (NCBI) | 9748 |
| RRID | AB_3672708 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H2G2 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SLK, also named as KIAA0204 and STK2, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and STE20 subfamily. SLK plays an important role in cell proliferation and cytoskeletal remodeling. In fibroblasts, SLK has been shown to respond to FAK/Src signaling and regulate focal adhesion turnover through Paxillin phosphorylation. Full-length SLK (~220 kDa) has also been shown to be essential for embryonic development. In myoblasts, the overexpression of a dominant negative SLK is sufficient to block myoblast fusion. (PMID: 28153048, PMID: 25882817, PMID: 23815977)
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 SLK antibody CL488-19743 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
