验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, human brain tissue, MCF-7 cells, mouse liver tissue, HeLa cells, THP-1 cells |
| Positive IHC detected in | human stomach cancer tissue, mouse heart tissue, mouse stomach tissue, rat heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
产品信息
19743-1-AP targets SLK in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | STE20-like kinase (yeast) |
| 别名 | CTCL tumor antigen se20 9, CTCL tumor antigen se20-9, EC:2.7.11.1, hSLK, KIAA0204 |
| 计算分子量 | 143 kDa |
| 观测分子量 | 143-160 kDa, 210-220 kDa |
| GenBank蛋白编号 | NM_014720 |
| 基因名称 | SLK |
| Gene ID (NCBI) | 9748 |
| RRID | AB_10644278 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H2G2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SLK, also named as KIAA0204 and STK2, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and STE20 subfamily. SLK plays an important role in cell proliferation and cytoskeletal remodeling. In fibroblasts, SLK has been shown to respond to FAK/Src signaling and regulate focal adhesion turnover through Paxillin phosphorylation. Full-length SLK (~220 kDa) has also been shown to be essential for embryonic development. In myoblasts, the overexpression of a dominant negative SLK is sufficient to block myoblast fusion. (PMID: 28153048, PMID: 25882817, PMID: 23815977)
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for SLK antibody 19743-1-AP | Download protocol |
| IHC protocol for SLK antibody 19743-1-AP | Download protocol |
| WB protocol for SLK antibody 19743-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Res Exon Skipping of Ste20-Like Kinase Enhances Glycolysis and Tumor Progression by Activating Enolase 1-Mediated Phosphoenolpyruvate Production | ||
Cell Mol Biol Lett Targeting SLK protects against cerebral ischemia-reperfusion injury by regulating USP8-mediated HIF-1α stabilization and RhoA/ROCK activation. | ||
Biochem Pharmacol Exon 13 skipping mediated by HNRNPL facilitates truncated SLK-induced metastasis in hepatocellular carcinoma. | ||
Biomedicines Estrogen Promotes Endometrial Cancer Development by Modulating ZNF626, SLK, and RFWD3 Gene Expression and Inducing Immune Inflammatory Changes | ||
J Med Virol LINC01012 Drives Hepatitis B-Related Hepatocellular Carcinoma Progression Through HNRNPL-Mediated Stabilization of Serine/Threonine-Protein Kinase-S. | ||
Neurol Res ITGA5 drives glioblastoma progression through SLK-mediated activation of the PI3K-Akt pathway |
