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验证数据展示

  • IHC staining of human small intestine using 27575-1-AP

    IHC staining of human small intestine using 27575-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 27575-1-AP (SLC6A19 antibody) at dilution of 1:400 (under 40x lens).

  • WB analysis using 27575-1-AP

    WB analysis using 27575-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27575-1-AP (SLC6A19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis using 27575-1-AP

    WB analysis using 27575-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27575-1-AP (SLC6A19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human small intestine using 27575-1-AP

    IHC staining of human small intestine using 27575-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 27575-1-AP (SLC6A19 antibody) at dilution of 1:800 (under 10x lens).

  • IHC staining of human small intestine using 27575-1-AP

    IHC staining of human small intestine using 27575-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 27575-1-AP (SLC6A19 antibody) at dilution of 1:800 (under 40x lens).

SLC6A19 Polyclonal antibody

SLC6A19 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27575-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

B0AT1, HND, Sodium-dependent neutral amino acid transporter B(0)AT1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inCaco-2 cells, mouse kidney tissue, PC-3 cells, mouse small intestine tissue
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27575-1-AP targets SLC6A19 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag24362

Product name: Recombinant human SLC6A19 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 295-403 aa of BC148801

Sequence: VHNNCEKDSVIVSIINGFTSVYVAIVVYSVIGFRATQRYDDCFSTNILTLINGFDLPEGNVTQENFVDMQQRCNASDPAAYAQLVFQTCDINAFLSEAVEGTGLAFIVF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 6 (neutral amino acid transporter), member 19
别名 B0AT1, HND, Sodium-dependent neutral amino acid transporter B(0)AT1
计算分子量 634 aa, 71 kDa
观测分子量71 kDa
GenBank蛋白编号BC148801
基因名称 SLC6A19
Gene ID (NCBI) 340024
RRIDAB_3085970
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ695T7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC6A19, also named as B0AT1, encodes system B(0) transmembrane protein that mediates epithelial resorption of neutral amino acids across the apical membrane in kidney and intestine (PubMed: 15044460). The protein contains 634 amino acids and has 12 predicted transmembrane regions with highly expression in human kidney and small intestine and minimal in pancreas (PubMed: 15286787). The expression of SLC6A19 in intestinal cells depends on angiotensin converting enzyme 2 which is the cellular receptor for SARS-CoV-2 (PubMed: 32132184). Mutations in SLC6A19 cause Hartnup disease (PMID: 15286787, PMID: 15286788).

实验方案

Product Specific Protocols
IHC protocol for SLC6A19 antibody 27575-1-APDownload protocol
WB protocol for SLC6A19 antibody 27575-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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