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CoraLite® Plus 488-conjugated SLC6A12 Monoclonal antibody

SLC6A12 Monoclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2C1G4

Cat No : CL488-67700

Print datasheet

Synonyms

BGT 1, BGT1, FLJ38727, SLC6A12

验证数据展示

  • IF Staining of Caco-2 using CL488-67700

    IF Staining of Caco-2 using CL488-67700

    Immunofluorescent analysis of (-20°C Ethanol) fixed Caco-2 cells using CoraLite® Plus 488 SLC6A12 antibody (CL488-67700, Clone: 2C1G4 ) at dilution of 1:200.

  • FC experiment of MDA-MB-231 using CL488-67700

    FC experiment of MDA-MB-231 using CL488-67700

    1X10^6 MDA-MB-231 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human SLC6A12 (CL488-67700, Clone:2C1G4) (red), or 0.8 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inCaco-2 cells
Positive FC detected inMDA-MB-231 cells
For other applications, we recommend the unconjugated version of this antibody, 67700-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67700 targets SLC6A12 in IF, FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity Human, Mouse, Rat
Immunogen SLC6A12 fusion protein Ag18962 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name solute carrier family 6 (neurotransmitter transporter, betaine/GABA), member 12
Synonyms BGT 1, BGT1, FLJ38727, SLC6A12
Calculated Molecular Weight 614 aa, 69 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC126215
Gene Symbol SLC6A12
Gene ID (NCBI) 6539
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP48065
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 SLC6A12 antibody CL488-67700Download protocol
FC protocol for CL Plus 488 SLC6A12 antibody CL488-67700Download protocol
Standard Protocols
Click here to view our Standard Protocols