验证数据展示
经过测试的应用
| Positive WB detected in | L02 cells, rat liver tissue, HSC-T6 cells, HuH-7 cells, HepG2 cells, mouse hepatocytes |
| Positive IF/ICC detected in | Caco-2 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
产品信息
67700-1-Ig targets SLC6A12 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human, Mouse, Rat |
| 文献引用反应性 | human, mouse, canine |
| 免疫原 |
CatNo: Ag18962 Product name: Recombinant human SLC6A12 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 548-614 aa of BC126215 Sequence: VCVPLFVVITLLKTRGPFRKRLRQLITPDSSLPQPKQHPCLDGSAGRNFGPSPTREGLIAGEKETHL 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | solute carrier family 6 (neurotransmitter transporter, betaine/GABA), member 12 |
| 别名 | BGT 1, BGT1, FLJ38727, SLC6A12 |
| 计算分子量 | 614 aa, 69 kDa |
| 观测分子量 | 70 kDa |
| GenBank蛋白编号 | BC126215 |
| 基因名称 | SLC6A12 |
| Gene ID (NCBI) | 6539 |
| RRID | AB_2882892 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P48065 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for SLC6A12 antibody 67700-1-Ig | Download protocol |
| WB protocol for SLC6A12 antibody 67700-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Am J Physiol Renal Physiol Mutation of a single threonine in the cytoplasmic NH2 terminus disrupts trafficking of renal betaine-GABA transporter 1 during hypertonic stress. | ||
Int J Biol Sci Sodium chloride promotes macrophage pyroptosis and aggravates rheumatoid arthritis by activating SGK1 through GABA receptors Slc6a12 | ||
Dev Cell The de novo synthesis of GABA and its gene regulatory function control hepatocellular carcinoma metastasis |
