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验证数据展示

  • FC experiment of A431 using CL488-68025

    FC experiment of A431 using CL488-68025

    1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human SLC39A3 (CL488-68025, Clone:1A11F6) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

CoraLite® Plus 488-conjugated SLC39A3 Monoclonal antibody

SLC39A3 Monoclonal Antibody for FC (Intra)
Cat No. CL488-68025

产品说明书

CloneNo. 1A11F6

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

FC (Intra)

别名

Zinc transporter ZIP3, ZIP 3, ZIP3, ZIP-3, Zrt and Irt like protein 3

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive FC (Intra) detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy® SLC39A3 Ready-To-Use IHC Kit. SLC39A3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68025-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68025 targets SLC39A3 in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原

CatNo: Ag14289

Product name: Recombinant human SLC39A3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 96-180 aa of BC005869

Sequence: FMTVFLEQLILTFRKEKPSFIDLETFNAGSDVGSDSEYESPFMGGARGHALYVEPHGHGPSLSVQGLSRASPVRLLSLAFALSAH

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 solute carrier family 39 (zinc transporter), member 3
别名 Zinc transporter ZIP3, ZIP 3, ZIP3, ZIP-3, Zrt and Irt like protein 3
计算分子量 314 aa, 34 kDa
观测分子量32 kDa
GenBank蛋白编号BC005869
基因名称 SLC39A3
Gene ID (NCBI) 29985
RRIDAB_2923863
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9BRY0
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC39A3, also named as ZIP3, belongs to the ZIP transporter (TC 2.A.5) family. SLC39A3 is a Zn importer that acts as a zinc-influx transporter. SLC39A3 is highly expressed in a cell-type-specific manner in ovaries, testes, and the developing nervous system with limited expression in the small intestinal crypt cells, kidney glomerulus, and discrete regions of the liver. SLC39A3 is also expressed in highly specialized, hormonally responsive secretory tissues, such as the pancreas, prostate, and mammary gland (PMID: 19458277). SLC39A3 has 2 isoforms with the molecular mass of 11 and 34 kDa.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 SLC39A3 antibody CL488-68025Download protocol
Standard Protocols
Click here to view our Standard Protocols
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