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验证数据展示

  • WB analysis of DU 145 using 83527-3-RR

    WB analysis of DU 145 using 83527-3-RR

    DU 145 cells were subjected to SDS PAGE followed by western blot with 83527-3-RR (SLC35B4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using 83527-3-RR

    FC experiment of HepG2 using 83527-3-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Slc35b4 Recombinant Antibody (83527-3-RR, Clone:240332F10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83527-3-RR

    Affinity and Kinetic Characterization of 83527-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83527-3-RR against Human SLC35B4 were performed. The affinity constant is below 1 pM.

SLC35B4 Recombinant antibody

SLC35B4 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83527-3-RR

产品说明书

CloneNo. 240332F10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

YEA4, YEA, UDP-xylose and UDP-N-acetylglucosamine transporter, PSEC0055, Nucleotide sugar transporter SLC35B4

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inDU 145 cells
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83527-3-RR targets SLC35B4 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 SLC35B4 fusion protein Ag22669 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 solute carrier family 35, member B4
别名 YEA4, YEA, UDP-xylose and UDP-N-acetylglucosamine transporter, PSEC0055, Nucleotide sugar transporter SLC35B4
观测分子量35-37 kDa
GenBank蛋白编号BC008413
基因名称 SLC35B4
Gene ID (NCBI) 84912
RRIDAB_3671154
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ969S0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC35B4 (solute carrier family 35 member B4), also known as YEA. It is expected to be located in the endoplasmic reticulum membrane, which is ubiquitinated in placenta and brain. SLC35B4 is a glycosyltransferase, which transports nucleotide sugars from the cytoplasm where they are synthesized, to the Golgi apparatus where they are utilized in the synthesis of glycoproteins, glycolipids, and proteoglycans (PMID: 15911612). Belongs to the nucleotide-sugar transporter family. SLC35B subfamily. The molecular weight of SLC35B4 is 37 kDa.

实验方案

Product Specific Protocols
WB protocol for SLC35B4 antibody 83527-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols