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验证数据展示

  • WB analysis of DU 145 using 83527-3-RR (same clone as 83527-3-PBS)

    WB analysis of DU 145 using 83527-3-RR (same clone as 83527-3-PBS)

    DU 145 cells were subjected to SDS PAGE followed by western blot with 83527-3-RR (SLC35B4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83527-3-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 83527-3-RR (same clone as 83527-3-PBS)

    FC experiment of HepG2 using 83527-3-RR (same clone as 83527-3-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Slc35b4 Recombinant Antibody (83527-3-RR, Clone:240332F10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83527-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00497-1

    Cytometric bead array standard curve of MP00497-1

    Cytometric bead array standard curve of MP00497-1, SLC35B4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83527-3-PBS. Detection antibody: 83527-2-PBS. Standard: Ag22669. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 83527-3-RR

    Affinity and Kinetic Characterization of 83527-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83527-3-RR against Human SLC35B4 were performed. The affinity constant is below 1 pM.

SLC35B4 Recombinant antibody, PBS Only (Capture)

SLC35B4 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA
Cat No. 83527-3-PBS

产品说明书

CloneNo. 240332F10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Cytometric bead array, Indirect ELISA

别名

YEA4, YEA, UDP-xylose and UDP-N-acetylglucosamine transporter, PSEC0055, Nucleotide sugar transporter SLC35B4

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83527-3-PBS targets SLC35B4 as part of a matched antibody pair:

MP00497-1: 83527-3-PBS capture and 83527-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 SLC35B4 fusion protein Ag22669 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 solute carrier family 35, member B4
别名 YEA4, YEA, UDP-xylose and UDP-N-acetylglucosamine transporter, PSEC0055, Nucleotide sugar transporter SLC35B4
GenBank蛋白编号BC008413
基因名称 SLC35B4
Gene ID (NCBI) 84912
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ969S0
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C