• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • FC experiment of NIH/3T3 using CL488-66367

    FC experiment of NIH/3T3 using CL488-66367

    1X10^6 NIH/3T3 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human SKAP2 (CL488-66367, Clone:2G3G9) (red), or 0.4 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of NIH/3T3 using CL488-66367

    IF Staining of NIH/3T3 using CL488-66367

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CoraLite® Plus 488 SKAP2 antibody (CL488-66367, Clone: 2G3G9 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated SKAP2 Monoclonal antibody

SKAP2 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-66367

产品说明书

CloneNo. 2G3G9

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

IF/ICC, FC (Intra)

别名

Retinoic acid-induced protein 70, RA70, Pyk2/RAFTK-associated protein, Pyk2/RAFTK associated protein, PRAP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive IF/ICC detected inNIH/3T3 cells
Positive FC (Intra) detected inNIH/3T3 cells
For other applications, we recommend the unconjugated version of this antibody, 66367-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66367 targets SKAP2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat, pig
免疫原 SKAP2 fusion protein Ag3615 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 src kinase associated phosphoprotein 2
别名 Retinoic acid-induced protein 70, RA70, Pyk2/RAFTK-associated protein, Pyk2/RAFTK associated protein, PRAP
计算分子量 41 kDa
观测分子量 55 kDa
GenBank蛋白编号BC036044
基因名称 SKAP2
Gene ID (NCBI) 8935
RRIDAB_3084239
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO75563
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SKAP2 (src kinase associated phosphoprotein 2), also named PRAP, contains PH domain and SH3 domain. It is localized in cytoplasm and was expressed in ubiquitously in various tissues including lung, skeletal muscle, and spleen, but not detectable in thymus and T cell lymphoma cells. SKAP2 may negatively regulate alpha-synuclein phosphorylation following cell stress. SKAP2 may be involved in B-cell and macrophage adhesion processes by coupling the B-cell receptor (BCR) to integrin activation.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 SKAP2 antibody CL488-66367Download protocol
FC protocol for CL Plus 488 SKAP2 antibody CL488-66367Download protocol
Standard Protocols
Click here to view our Standard Protocols