CoraLite® Plus 488-conjugated SKAP2 Monoclonal antibody
SKAP2 Monoclonal Antibody for IF/ICC, FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
human, pig, rat, mouse
Applications
IF/ICC, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2G3G9
验证数据展示
经过测试的应用
Positive IF detected in | NIH/3T3 cells |
Positive FC detected in | NIH/3T3 cells |
For other applications, we recommend the unconjugated version of this antibody, 66367-1-Ig
推荐稀释比
Application | Dilution |
---|---|
Immunofluorescence (IF) | IF : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.80 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-66367 targets SKAP2 in IF, FC (Intra) applications and shows reactivity with human, pig, rat, mouse samples.
Tested Applications | IF/ICC, FC (Intra) |
Tested Reactivity | human, pig, rat, mouse |
Immunogen | SKAP2 fusion protein Ag3615 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | src kinase associated phosphoprotein 2 |
Synonyms | PRAP, Pyk2/RAFTK associated protein, RA70, SAPS, SCAP2, SKAP 55HOM, SKAP HOM, SKAP2, SKAP55 homolog, SKAP55R |
Calculated Molecular Weight | 41 kDa |
Observed Molecular Weight | 55 kDa |
GenBank Accession Number | BC036044 |
Gene Symbol | SKAP2 |
Gene ID (NCBI) | 8935 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | O75563 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SKAP2 (src kinase associated phosphoprotein 2), also named PRAP, contains PH domain and SH3 domain. It is localized in cytoplasm and was expressed in ubiquitously in various tissues including lung, skeletal muscle, and spleen, but not detectable in thymus and T cell lymphoma cells. SKAP2 may negatively regulate alpha-synuclein phosphorylation following cell stress. SKAP2 may be involved in B-cell and macrophage adhesion processes by coupling the B-cell receptor (BCR) to integrin activation.
实验方案
Product Specific Protocols | |
---|---|
IF protocol for CL Plus 488 SKAP2 antibody CL488-66367 | Download protocol |
FC protocol for CL Plus 488 SKAP2 antibody CL488-66367 | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |