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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 66347-1-Ig (same clone as 66347-1-PBS)

    WB analysis using 66347-1-Ig (same clone as 66347-1-PBS)

    Various cells were subjected to SDS PAGE followed by western blot with 66347-1-Ig (SIX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66347-1-PBS in a different storage buffer formulation.

  • WB analysis using 66347-1-Ig (same clone as 66347-1-PBS)

    WB analysis using 66347-1-Ig (same clone as 66347-1-PBS)

    Various tissues were subjected to SDS PAGE followed by western blot with 66347-1-Ig (SIX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66347-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse embryo using 66347-1-Ig (same clone as 66347-1-PBS)

    IHC staining of mouse embryo using 66347-1-Ig (same clone as 66347-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 66347-1-Ig (SIX2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66347-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66347-1-Ig (same clone as 66347-1-PBS)

    IF Staining of HeLa using 66347-1-Ig (same clone as 66347-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SIX2 antibody (66347-1-Ig, Clone: 1F9G11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66347-1-PBS in a different storage buffer formulation.

SIX2 Monoclonal antibody, PBS Only

SIX2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66347-1-PBS

产品说明书

CloneNo. 1F9G11

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat, pig, canine

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Homeobox protein SIX2, Sine oculis homeobox homolog 2, SIX homeobox 2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66347-1-PBS targets SIX2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, canine samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig, canine
免疫原 SIX2 fusion protein Ag2124 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 SIX homeobox 2
别名 Homeobox protein SIX2, Sine oculis homeobox homolog 2, SIX homeobox 2
计算分子量 291 aa, 32 kDa
观测分子量 37 kDa
GenBank蛋白编号BC024033
基因名称 SIX2
Gene ID (NCBI) 10736
RRIDAB_2881727
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NPC8
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The SIX proteins (sine oculis) are a family of homeodomain transcription factors that share a conserved DNA binding domain. Six members (Six1-Six6) of the Six gene family have been identified in mice and humans. SIX2, containing one homeobox DNA-binding domain, is highly expressed in fetal tissues but expression is limited in adult tissues. SIX2 may be involved in limb tendon and ligament development[PMID:21420949]. It has been previously shown that SIX2 is expressed in developing mesenchymal tissue including head and urogenital system at the time of overt midfacial and renal differentiation[PMID: 22282599].