验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | A431 cells |
| Positive FC detected in | A431 cells |
For other applications, we recommend the unconjugated version of this antibody, 67197-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67197 targets SIN3A in FC (Intra) applications and shows reactivity with Human, mouse, rat samples.
| 经测试应用 | FC (Intra) Application Description |
| 经测试反应性 | Human, mouse, rat |
| 免疫原 |
CatNo: Ag28865 Product name: Recombinant human SIN3A protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-156 aa of BC066364 Sequence: MKRRLDDQESPVYAAQQRRIPGSTEAFPHQHRVLAPAPPVYEAVSETMQSATGIQYSVTPSYQVSAMPQSSGSHGPAIAAVHSSHHHPTAVQPHGGQVVQSHAHPAPPVAPVQGQQQFQRLKVVFSSFSWVKKFIFSRKYWLQLVGCGGTTSNLRY 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | SIN3 homolog A, transcription regulator (yeast) |
| 别名 | SIN3A, 2B12E8, KIAA0700 |
| 计算分子量 | 145 kDa |
| 观测分子量 | 145-150 kDa |
| GenBank蛋白编号 | BC066364 |
| 基因名称 | SIN3A |
| Gene ID (NCBI) | 25942 |
| RRID | AB_3084323 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q96ST3 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 SIN3A antibody CL488-67197 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
