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SIN3A Monoclonal antibody

SIN3A Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2B12E8

Cat No : 67197-1-Ig

Print datasheet

Synonyms

DKFZp434K2235, FLJ90319, KIAA0700, SIN3A

验证数据展示

  • WB analysis using 67197-1-Ig

    WB analysis using 67197-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67197-1-Ig (SIN3A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 67197-1-Ig

    IF Staining of U2OS using 67197-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SIN3A antibody (67197-1-Ig, Clone: 2B12E8 ) at dilution of 1:2500 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

经过测试的应用

Positive WB detected inHeLa cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, HEK-293 cells, MCF-7 cells, Jurkat cells
Positive IF detected inU2OS cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)IF : 1:1250-1:5000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67197-1-Ig targets SIN3A in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IF, ELISA
Tested Reactivity Human, mouse, rat
Immunogen SIN3A fusion protein Ag28865 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name SIN3 homolog A, transcription regulator (yeast)
Synonyms DKFZp434K2235, FLJ90319, KIAA0700, SIN3A
Calculated Molecular Weight 145 kDa
Observed Molecular Weight 145-150 kDa
GenBank Accession NumberBC066364
Gene Symbol SIN3A
Gene ID (NCBI) 25942
RRIDAB_2882490
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ96ST3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SIN3A antibody 67197-1-IgDownload protocol
IF protocol for SIN3A antibody 67197-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols